The irreversible inactivation of two copper-dependent monooxygenases by sulfite

peptidylglycine α-amidating enzyme and dopamine β-monooxygenase

David J. Merkler, Raviraj Kulathila, Wilson A. Francisco, David E. Ash, Joseph Bell

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Peptidylglycine α-amidating enzyme (α-AE) and dopamine β-monooxygenase (DβM), two copper-dependent monooxygenases that have catalytic and structural similarities, are irreversibly inactivated by sodium sulfite in a time- and concentration-dependent manner. Studies with α-AE show that the sulfite-mediated inactivation is dependent on the presence of redox active transition metals free in solution, with Cu(II) being the most effective in supporting the inactivation reaction. Sulfite inactivation of α-AE is specific for the monooxygenase reaction of this bifunctional enzyme and amidated peptides provide protection against the inactivation. Consequently, the sulfite-mediated inactivation of α-AE and DβM most likely results from the transition metal-catalyzed oxidation of sulfite to the sulfite radical, SO3 -.

Original languageEnglish (US)
Pages (from-to)165-169
Number of pages5
JournalFEBS Letters
Volume366
Issue number2-3
DOIs
StatePublished - Jun 12 1995
Externally publishedYes

Fingerprint

Sulfites
Mixed Function Oxygenases
Copper
Dopamine
Enzymes
Transition metals
Metals
Oxidation-Reduction
peptidylglycine monooxygenase
Oxidation
Peptides

Keywords

  • Copper-dependent monooxygenase
  • Dopamine hydroxylation
  • Peptide α-amidation
  • Sulfite-mediated inactivation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

The irreversible inactivation of two copper-dependent monooxygenases by sulfite : peptidylglycine α-amidating enzyme and dopamine β-monooxygenase. / Merkler, David J.; Kulathila, Raviraj; Francisco, Wilson A.; Ash, David E.; Bell, Joseph.

In: FEBS Letters, Vol. 366, No. 2-3, 12.06.1995, p. 165-169.

Research output: Contribution to journalArticle

Merkler, David J. ; Kulathila, Raviraj ; Francisco, Wilson A. ; Ash, David E. ; Bell, Joseph. / The irreversible inactivation of two copper-dependent monooxygenases by sulfite : peptidylglycine α-amidating enzyme and dopamine β-monooxygenase. In: FEBS Letters. 1995 ; Vol. 366, No. 2-3. pp. 165-169.
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