The involvement of photosystem II-generated H2O2 in photoinhibition

Ryan L. Bradley; Krista M. Long; Wayne Frasch

doi:10.1016/0014-5793(91)80975-9

The involvement of photosystem II-generated H₂O₂ in photoinhibition

Ryan L. Bradley, Krista M. Long, Wayne Frasch

Life Sciences, School of (SOLS)

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The involvement of H₂O₂ generated by photosystem II (PSII) in the process of photoinhibition of thylakoids with a functional oxygen-evolving complex (OEC) was investigated. The rate of photoinhibition was decreased to the rate of loss of activity in the dark when bovine Fe-catalase was present during the photoinhibitory illumination. Photoinhibition was accelerated for both Cl^--depleted and Cl^--sufficient thylakoids when KCN was present to inhibit the thylakoid-bound Fe-catalase. We propose that these preparations become photoinhibed by reactions with H₂O₂ produced via oxidation of water by the Cl^--depleted OEC and by reduction of O₂ at the Q_B site when PSII is illuminated without an electron acceptor.

Original language	English (US)
Pages (from-to)	209-213
Number of pages	5
Journal	FEBS Letters
Volume	286
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(91)80975-9
State	Published - Jul 29 1991

Keywords

D1 protein
HO
Photoinhibition
Photosystem II

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(91)80975-9

Cite this

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title = "The involvement of photosystem II-generated H2O2 in photoinhibition",

abstract = "The involvement of H2O2 generated by photosystem II (PSII) in the process of photoinhibition of thylakoids with a functional oxygen-evolving complex (OEC) was investigated. The rate of photoinhibition was decreased to the rate of loss of activity in the dark when bovine Fe-catalase was present during the photoinhibitory illumination. Photoinhibition was accelerated for both Cl--depleted and Cl--sufficient thylakoids when KCN was present to inhibit the thylakoid-bound Fe-catalase. We propose that these preparations become photoinhibed by reactions with H2O2 produced via oxidation of water by the Cl--depleted OEC and by reduction of O2 at the QB site when PSII is illuminated without an electron acceptor.",

keywords = "D1 protein, HO, Photoinhibition, Photosystem II",

author = "Bradley, {Ryan L.} and Long, {Krista M.} and Wayne Frasch",

note = "Funding Information: Acknow/e&ernen/d: The authors want to thank Stan Williams, Almaz Gebregiorgisa nd Alyson Roskelleyf or their superb technical assistanceT. his project was supportedb y a ResearchI ncentiveA ward from ASU to WDF and is publicationn o. 73 from the Arizona State University Center for the Study of Early Events in Photosynthesis. The Center is funded by the U.S. Departmento f Energy Grant DE-FGOt-8&?ER/3969a s part of the USDA/DOE/NSF Plant Science Center Program.",

year = "1991",

month = jul,

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doi = "10.1016/0014-5793(91)80975-9",

language = "English (US)",

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pages = "209--213",

journal = "FEBS Letters",

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AU - Bradley, Ryan L.

AU - Long, Krista M.

AU - Frasch, Wayne

N1 - Funding Information: Acknow/e&ernen/d: The authors want to thank Stan Williams, Almaz Gebregiorgisa nd Alyson Roskelleyf or their superb technical assistanceT. his project was supportedb y a ResearchI ncentiveA ward from ASU to WDF and is publicationn o. 73 from the Arizona State University Center for the Study of Early Events in Photosynthesis. The Center is funded by the U.S. Departmento f Energy Grant DE-FGOt-8&?ER/3969a s part of the USDA/DOE/NSF Plant Science Center Program.

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N2 - The involvement of H2O2 generated by photosystem II (PSII) in the process of photoinhibition of thylakoids with a functional oxygen-evolving complex (OEC) was investigated. The rate of photoinhibition was decreased to the rate of loss of activity in the dark when bovine Fe-catalase was present during the photoinhibitory illumination. Photoinhibition was accelerated for both Cl--depleted and Cl--sufficient thylakoids when KCN was present to inhibit the thylakoid-bound Fe-catalase. We propose that these preparations become photoinhibed by reactions with H2O2 produced via oxidation of water by the Cl--depleted OEC and by reduction of O2 at the QB site when PSII is illuminated without an electron acceptor.

AB - The involvement of H2O2 generated by photosystem II (PSII) in the process of photoinhibition of thylakoids with a functional oxygen-evolving complex (OEC) was investigated. The rate of photoinhibition was decreased to the rate of loss of activity in the dark when bovine Fe-catalase was present during the photoinhibitory illumination. Photoinhibition was accelerated for both Cl--depleted and Cl--sufficient thylakoids when KCN was present to inhibit the thylakoid-bound Fe-catalase. We propose that these preparations become photoinhibed by reactions with H2O2 produced via oxidation of water by the Cl--depleted OEC and by reduction of O2 at the QB site when PSII is illuminated without an electron acceptor.

KW - D1 protein

KW - HO

KW - Photoinhibition

KW - Photosystem II

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