TY - JOUR
T1 - The Independent and Shared Transcriptomic Response to UVA, UVB and Oxidative Stress in the Cyanobacterium Nostoc punctiforme ATCC 29133
AU - Soule, Tanya
AU - Ferreira, Daniela
AU - Lothamer, Justin
AU - Garcia-Pichel, Ferran
N1 - Funding Information:
We wish to thank Scott Bingham of the Arizona State University DNA Lab for assistance processing the samples. This work was funded as a Research Opportunity Award (ROA) from the parent grant 1158551.
Funding Information:
We wish to thank Scott Bingham of the Arizona State University DNA Lab for assistance processing the samples. This work was funded as a Research Opportunity Award (ROA) from the parent grant 1158551.
Publisher Copyright:
© 2021 American Society for Photobiology.
PY - 2021/9/1
Y1 - 2021/9/1
N2 - Research on the UVA, UVB and oxidative (as reactive oxygen species, ROS) stress response in cyanobacteria has typically focused on each individual stress condition, with limited studies addressing the intersection. Here, we evaluated the transcriptomic responses of the model cyanobacterium Nostoc punctiforme after exposure to each of these conditions. Overall, response to UVA was characterized by more gene down-regulation than the UVB or ROS response, although UVB affected over fourfold more genes than UVA or ROS. Regarding expression patterns, responses to UVA and ROS were more similar and differentiated from those to UVB. For example, genes involved in ROS metabolism were up-regulated under both UVA and ROS. However, when it came to RNA and protein metabolism, there were more up-regulated genes under UVB and ROS compared to UVA. This suggests that the response to UVB and ROS is more active than the response to UVA, which stimulated more genes in secondary metabolism. Histidine kinases and response regulators were often differentially expressed, demonstrating that regulatory systems were at the base of the patterns. This study provides background for future studies targeting different genes, proteins and systems sensitive to these conditions. It also highlights the significance of considering multiple stress conditions.
AB - Research on the UVA, UVB and oxidative (as reactive oxygen species, ROS) stress response in cyanobacteria has typically focused on each individual stress condition, with limited studies addressing the intersection. Here, we evaluated the transcriptomic responses of the model cyanobacterium Nostoc punctiforme after exposure to each of these conditions. Overall, response to UVA was characterized by more gene down-regulation than the UVB or ROS response, although UVB affected over fourfold more genes than UVA or ROS. Regarding expression patterns, responses to UVA and ROS were more similar and differentiated from those to UVB. For example, genes involved in ROS metabolism were up-regulated under both UVA and ROS. However, when it came to RNA and protein metabolism, there were more up-regulated genes under UVB and ROS compared to UVA. This suggests that the response to UVB and ROS is more active than the response to UVA, which stimulated more genes in secondary metabolism. Histidine kinases and response regulators were often differentially expressed, demonstrating that regulatory systems were at the base of the patterns. This study provides background for future studies targeting different genes, proteins and systems sensitive to these conditions. It also highlights the significance of considering multiple stress conditions.
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U2 - 10.1111/php.13444
DO - 10.1111/php.13444
M3 - Article
C2 - 33955032
AN - SCOPUS:85106314738
SN - 0031-8655
VL - 97
SP - 1063
EP - 1071
JO - Photochemistry and photobiology
JF - Photochemistry and photobiology
IS - 5
ER -