The importance of organism density and co-occurring organisms in biases associated with molecular studies of marine protist diversity

Jessica A. Amacher, Charles W. Baysinger, Susanne Neuer

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

DNA-based molecular methods such as clone libraries constructed of 18S rRNA amplicons have been increasingly employed in surveys of marine protist communities but are known to have underlying biases, and especially haptophytes are known to be underestimated in eukaryotic clone libraries. Using controlled mixtures of laboratory strains, we show that the recovery of organisms in clone libraries is both influenced by their concentration in the environment as well as bias exerted by certain co-occurring organisms.

Original languageEnglish (US)
Pages (from-to)1762-1766
Number of pages5
JournalJournal of Plankton Research
Volume33
Issue number11
DOIs
StatePublished - Nov 1 2011

Keywords

  • 18S rRNA gene
  • bias
  • microbial ecology
  • protists
  • universal primers

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology

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