The identification and localization of 33 pea chloroplast transcription initiation sites

Neal Woodbury, Michael Dobres, William F. Thompson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We have used a novel approach to produce a comprehensive transcription initiation map of the pea chloroplast genome. Sites were mapped by measuring the ability of DNA probes to protect 5′ ends of transcripts that have been capped in vitro. Using this approach, at least 33 probes appear to contain one or more transcription start sites. A more precise location of some of these sites was obtained by hybrid selecting certain of these RNAs and determining their size both before and after RNase treatment. We have found at least one initiation site in front of every chloroplast gene cluster for which appropriate clones were available. In addition, we have found initiation sites within gene clusters previously shown to be co-transcribed. In one such case, we were able to locate a transcription start site for psbC within the coding sequence of psbD.

Original languageEnglish (US)
Pages (from-to)433-445
Number of pages13
JournalCurrent Genetics
Volume16
Issue number5-6
DOIs
StatePublished - Dec 1989

Fingerprint

Transcription Initiation Site
Peas
Chloroplasts
Multigene Family
Chloroplast Genes
Chloroplast Genome
DNA Probes
Ribonucleases
Clone Cells
RNA
In Vitro Techniques

Keywords

  • Chloroplast
  • Pea
  • Pisum sativum
  • Transcription initiation

ASJC Scopus subject areas

  • Genetics

Cite this

The identification and localization of 33 pea chloroplast transcription initiation sites. / Woodbury, Neal; Dobres, Michael; Thompson, William F.

In: Current Genetics, Vol. 16, No. 5-6, 12.1989, p. 433-445.

Research output: Contribution to journalArticle

Woodbury, Neal ; Dobres, Michael ; Thompson, William F. / The identification and localization of 33 pea chloroplast transcription initiation sites. In: Current Genetics. 1989 ; Vol. 16, No. 5-6. pp. 433-445.
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