The hyphal tip structure of Basidiobolus sp. A zygomycete fungus of uncertain phylogeny

Robert Roberson, Evonne Saucedo, Daniel Maclean, Jeff Propster, Brant Unger, Terrence A. Oneil, Kymia Parvanehgohar, Courtney Cavanaugh, David Lowry

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

To date, among the zygomycete fungi that have been examined, a Spitzenkörper has not been reported. In this paper, the cytoplasmic order of hyphal tip cells of Basidiobolus sp., a zygomycete genus of uncertain phylogeny, has been examined using light microscopy and transmission electron microscopy methods. With phase-contrast light optics, a phase-dark body was observed at the tips of growing hyphae of Basidiobolus sp. The hyphal apex also showed high affinity for FM4-64 labelling resulting in an intense fluorescence signal. The phase-dark inclusion exhibited independent motility within the hyphal apex and its presence and position were correlated to the rate and direction of hyphal growth. The hyphal apex of Basidiobolus sp. did not contain γ-tubulin. Ultrastructural observations revealed a dense cluster of vesicles at the hyphal apex. These results suggest that the growing hypha of Basidiobolus sp. contains a Spitzenkörper, a character generally attributed to members of the ascomycete and basidiomycete fungi and not to zygomycete fungi.

Original languageEnglish (US)
Pages (from-to)485-492
Number of pages8
JournalFungal Biology
Volume115
Issue number6
DOIs
StatePublished - Jun 2011

Keywords

  • Apical growth
  • Bioimaging
  • Hyphal structure

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Infectious Diseases

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