The heat released during catalytic turnover enhances the diffusion of an enzyme

Clement Riedel, Ronen Gabizon, Christian A.M. Wilson, Kambiz Hamadani, Konstantinos Tsekouras, Susan Marqusee, Steve Presse, Carlos Bustamante

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Recent studies have shown that the diffusivity of enzymes increases in a substrate-dependent manner during catalysis1,2. Although this observation has been reported and characterized for several different systems3-10, the precise origin of this phenomenon is unknown. Calorimetric methods are often used to determine enthalpies from enzyme-catalysed reactions and can therefore provide important insight into their reaction mechanisms11,12. The ensemble averages involved in traditional bulk calorimetry cannot probe the transient effects that the energy exchanged in a reaction may have on the catalyst. Here we obtain single-molecule fluorescence correlation spectroscopy data and analyse them within the framework of a stochastic theory to demonstrate a mechanistic link between the enhanced diffusion of a single enzyme molecule and the heat released in the reaction. We propose that the heat released during catalysis generates an asymmetric pressure wave that results in a differential stress at the protein-solvent interface that transiently displaces the centre-of-mass of the enzyme (chemoacoustic effect). This novel perspective on how enzymes respond to the energy released during catalysis suggests a possible effect of the heat of reaction on the structural integrity and internal degrees of freedom of the enzyme.

Original languageEnglish (US)
Pages (from-to)227-230
Number of pages4
JournalNature
Volume517
Issue number7533
DOIs
StatePublished - Jan 8 2015
Externally publishedYes

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Hot Temperature
Enzymes
Catalysis
Calorimetry
Fluorescence Spectrometry
Heat-Shock Proteins
Pressure

ASJC Scopus subject areas

  • General

Cite this

Riedel, C., Gabizon, R., Wilson, C. A. M., Hamadani, K., Tsekouras, K., Marqusee, S., ... Bustamante, C. (2015). The heat released during catalytic turnover enhances the diffusion of an enzyme. Nature, 517(7533), 227-230. https://doi.org/10.1038/nature14043

The heat released during catalytic turnover enhances the diffusion of an enzyme. / Riedel, Clement; Gabizon, Ronen; Wilson, Christian A.M.; Hamadani, Kambiz; Tsekouras, Konstantinos; Marqusee, Susan; Presse, Steve; Bustamante, Carlos.

In: Nature, Vol. 517, No. 7533, 08.01.2015, p. 227-230.

Research output: Contribution to journalArticle

Riedel, C, Gabizon, R, Wilson, CAM, Hamadani, K, Tsekouras, K, Marqusee, S, Presse, S & Bustamante, C 2015, 'The heat released during catalytic turnover enhances the diffusion of an enzyme', Nature, vol. 517, no. 7533, pp. 227-230. https://doi.org/10.1038/nature14043
Riedel C, Gabizon R, Wilson CAM, Hamadani K, Tsekouras K, Marqusee S et al. The heat released during catalytic turnover enhances the diffusion of an enzyme. Nature. 2015 Jan 8;517(7533):227-230. https://doi.org/10.1038/nature14043
Riedel, Clement ; Gabizon, Ronen ; Wilson, Christian A.M. ; Hamadani, Kambiz ; Tsekouras, Konstantinos ; Marqusee, Susan ; Presse, Steve ; Bustamante, Carlos. / The heat released during catalytic turnover enhances the diffusion of an enzyme. In: Nature. 2015 ; Vol. 517, No. 7533. pp. 227-230.
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