The growth and cytopathogenicity of virulent and attenuated strains of canine distemper virus in dog and ferret macrophages

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Abstract

The in vitro growth of virulent and attenuated strains of canine distemper virus in dog and ferret lung, liver, spleen and peritoneal macrophages was examined. All virus strains tested grew in each type of macrophage culture. Cytopathic effects, consisting of cytoplasmic and nuclear inclusion bodies and polykaryocytosis, were found in all virus-cell combinations. The polykaryocytes were formed both by cell fusion and from single cells by amitosis. Marked nuclear abnormalities were found in alveolar macrophages infected with virulent virus strains. The role of lysosomal enzymes in the development of these cytopathic effects was discussed.

Original languageEnglish (US)
JournalJournal of Comparative Pathology
Volume81
Issue number1
DOIs
StatePublished - 1971
Externally publishedYes

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Canine Distemper Virus
Ferrets
Canine distemper virus
cytopathogenicity
ferrets
macrophages
Macrophages
Dogs
Viruses
viruses
dogs
Growth
nuclear inclusions
cytoplasmic inclusions
Intranuclear Inclusion Bodies
cell fusion
Cell Fusion
Inclusion Bodies
Alveolar Macrophages
Peritoneal Macrophages

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

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abstract = "The in vitro growth of virulent and attenuated strains of canine distemper virus in dog and ferret lung, liver, spleen and peritoneal macrophages was examined. All virus strains tested grew in each type of macrophage culture. Cytopathic effects, consisting of cytoplasmic and nuclear inclusion bodies and polykaryocytosis, were found in all virus-cell combinations. The polykaryocytes were formed both by cell fusion and from single cells by amitosis. Marked nuclear abnormalities were found in alveolar macrophages infected with virulent virus strains. The role of lysosomal enzymes in the development of these cytopathic effects was discussed.",
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AB - The in vitro growth of virulent and attenuated strains of canine distemper virus in dog and ferret lung, liver, spleen and peritoneal macrophages was examined. All virus strains tested grew in each type of macrophage culture. Cytopathic effects, consisting of cytoplasmic and nuclear inclusion bodies and polykaryocytosis, were found in all virus-cell combinations. The polykaryocytes were formed both by cell fusion and from single cells by amitosis. Marked nuclear abnormalities were found in alveolar macrophages infected with virulent virus strains. The role of lysosomal enzymes in the development of these cytopathic effects was discussed.

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