Abstract
Mutations induced by pollutants may promote pathogen evolution, for example by accelerating mutations conferring antibiotic resistance. Generally, evaluating the genome-wide mutagenic effects of long-term sublethal pollutant exposure at single-nucleotide resolution is extremely difficult. To overcome this technical barrier, we use the mutation accumulation/whole-genome sequencing (MA/WGS) method as a mutagenicity test, to quantitatively evaluate genome-wide mutagenesis of Escherichia coli after long-term exposure to a wide gradient of the glyphosate-based herbicide (GBH) Roundup Concentrate Plus. The genome-wide mutation rate decreases as GBH concentration increases, suggesting that even long-term GBH exposure does not compromise the genome stability of bacteria.
Original language | English (US) |
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Pages (from-to) | 3331-3335 |
Number of pages | 5 |
Journal | G3: Genes, Genomes, Genetics |
Volume | 7 |
Issue number | 10 |
DOIs | |
State | Published - Oct 1 2017 |
Externally published | Yes |
Keywords
- Ecological dependence of mutations
- Environmental mutagenesis
- Evolutionary genomics
- Herbicide damage
- Mutagenicity test
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Genetics(clinical)