Abstract
Mutations induced by pollutants may promote pathogen evolution, for example by accelerating mutations conferring antibiotic resistance. Generally, evaluating the genome-wide mutagenic effects of long-term sublethal pollutant exposure at single-nucleotide resolution is extremely difficult. To overcome this technical barrier, we use the mutation accumulation/whole-genome sequencing (MA/WGS) method as a mutagenicity test, to quantitatively evaluate genome-wide mutagenesis of Escherichia coli after long-term exposure to a wide gradient of the glyphosate-based herbicide (GBH) Roundup Concentrate Plus. The genome-wide mutation rate decreases as GBH concentration increases, suggesting that even long-term GBH exposure does not compromise the genome stability of bacteria.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3331-3335 |
| Number of pages | 5 |
| Journal | G3: Genes, Genomes, Genetics |
| Volume | 7 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 1 2017 |
| Externally published | Yes |
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Keywords
- Ecological dependence of mutations
- Environmental mutagenesis
- Evolutionary genomics
- Herbicide damage
- Mutagenicity test
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Genetics(clinical)
Cite this
The glyphosate-based herbicide roundup does not elevate genome-wide mutagenesis of Escherichia coli. / Tincher, Clayton; Long, Hongan; Behringer, Megan; Walker, Noah; Lynch, Michael.
In: G3: Genes, Genomes, Genetics, Vol. 7, No. 10, 01.10.2017, p. 3331-3335.Research output: Contribution to journal › Article
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TY - JOUR
T1 - The glyphosate-based herbicide roundup does not elevate genome-wide mutagenesis of Escherichia coli
AU - Tincher, Clayton
AU - Long, Hongan
AU - Behringer, Megan
AU - Walker, Noah
AU - Lynch, Michael
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Mutations induced by pollutants may promote pathogen evolution, for example by accelerating mutations conferring antibiotic resistance. Generally, evaluating the genome-wide mutagenic effects of long-term sublethal pollutant exposure at single-nucleotide resolution is extremely difficult. To overcome this technical barrier, we use the mutation accumulation/whole-genome sequencing (MA/WGS) method as a mutagenicity test, to quantitatively evaluate genome-wide mutagenesis of Escherichia coli after long-term exposure to a wide gradient of the glyphosate-based herbicide (GBH) Roundup Concentrate Plus. The genome-wide mutation rate decreases as GBH concentration increases, suggesting that even long-term GBH exposure does not compromise the genome stability of bacteria.
AB - Mutations induced by pollutants may promote pathogen evolution, for example by accelerating mutations conferring antibiotic resistance. Generally, evaluating the genome-wide mutagenic effects of long-term sublethal pollutant exposure at single-nucleotide resolution is extremely difficult. To overcome this technical barrier, we use the mutation accumulation/whole-genome sequencing (MA/WGS) method as a mutagenicity test, to quantitatively evaluate genome-wide mutagenesis of Escherichia coli after long-term exposure to a wide gradient of the glyphosate-based herbicide (GBH) Roundup Concentrate Plus. The genome-wide mutation rate decreases as GBH concentration increases, suggesting that even long-term GBH exposure does not compromise the genome stability of bacteria.
KW - Ecological dependence of mutations
KW - Environmental mutagenesis
KW - Evolutionary genomics
KW - Herbicide damage
KW - Mutagenicity test
UR - http://www.scopus.com/inward/record.url?scp=85030624190&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85030624190&partnerID=8YFLogxK
U2 - 10.1534/g3.117.300133
DO - 10.1534/g3.117.300133
M3 - Article
VL - 7
SP - 3331
EP - 3335
JO - G3 (Bethesda, Md.)
JF - G3 (Bethesda, Md.)
SN - 2160-1836
IS - 10
ER -