The glyphosate-based herbicide roundup does not elevate genome-wide mutagenesis of Escherichia coli

Clayton Tincher, Hongan Long, Megan Behringer, Noah Walker, Michael Lynch

Research output: Contribution to journalArticle

1 Scopus citations


Mutations induced by pollutants may promote pathogen evolution, for example by accelerating mutations conferring antibiotic resistance. Generally, evaluating the genome-wide mutagenic effects of long-term sublethal pollutant exposure at single-nucleotide resolution is extremely difficult. To overcome this technical barrier, we use the mutation accumulation/whole-genome sequencing (MA/WGS) method as a mutagenicity test, to quantitatively evaluate genome-wide mutagenesis of Escherichia coli after long-term exposure to a wide gradient of the glyphosate-based herbicide (GBH) Roundup Concentrate Plus. The genome-wide mutation rate decreases as GBH concentration increases, suggesting that even long-term GBH exposure does not compromise the genome stability of bacteria.

Original languageEnglish (US)
Pages (from-to)3331-3335
Number of pages5
JournalG3: Genes, Genomes, Genetics
Issue number10
StatePublished - Oct 1 2017
Externally publishedYes



  • Ecological dependence of mutations
  • Environmental mutagenesis
  • Evolutionary genomics
  • Herbicide damage
  • Mutagenicity test

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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