The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus

W. Matthew Sattley, Michael T. Madigan, Wesley D. Swingley, Patricia C. Cheung, Kate M. Clocksin, Amber L. Conrad, Liza C. Dejesa, Barbara M. Honchak, Deborah O. Jung, Lauren E. Karbach, Ahmet Kurdoglu, Surobhi Lahiri, Stephen D. Mastrian, Lawrence E. Page, Heather L. Taylor, Zi T. Wang, Jason Raymond, Min Chen, Robert E. Blankenship, Jeffrey W. Touchman

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Despite the fact that heliobacteria are the only phototrophic representatives of the bacterial phylum Firmicutes, genomic analyses of these organisms have yet to be reported. Here we describe the complete sequence and analysis of the genome of Heliobacterium modesticaldum, a thermophilic species belonging to this unique group of phototrophs. The genome is a single 3.1-Mb circular chromosome containing 3,138 open reading frames. As suspected from physiological studies of heliobacteria that have failed to show photoautotrophic growth, genes encoding enzymes for known autotrophic pathways in other phototrophic organisms, including ribulose bisphosphate carboxylase (Calvin cycle), citrate lyase (reverse citric acid cycle), and malyl coenzyme A lyase (3-hydroxypropionate pathway), are not present in the H. modesticaldum genome. Thus, heliobacteria appear to be the only known anaerobic anoxygenic phototrophs that are not capable of autotrophy. Although for some cellular activities, such as nitrogen fixation, there is a full complement of genes in H. modesticaldum, other processes, including carbon metabolism and endosporulation, are more genetically streamlined than they are in most other low-G+C gram-positive bacteria. Moreover, several genes encoding photosynthetic functions in phototrophic purple bacteria are not present in the heliobacteria. In contrast to the nutritional flexibility of many anoxygenic phototrophs, the complete genome sequence of H. modesticaldum reveals an organism with a notable degree of metabolic specialization and genomic reduction.

Original languageEnglish (US)
Pages (from-to)4687-4696
Number of pages10
JournalJournal of Bacteriology
Volume190
Issue number13
DOIs
StatePublished - Jul 2008
Externally publishedYes

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Genome
citrate (pro-3S)-lyase
Autotrophic Processes
Genes
Ribulose-Bisphosphate Carboxylase
Proteobacteria
Nitrogen Fixation
Chromosomes, Human, Pair 3
Citric Acid Cycle
Photosynthesis
Gram-Positive Bacteria
Open Reading Frames
Sequence Analysis
Carbon
Firmicutes
Enzymes
Growth
malyl-CoA lyase

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Sattley, W. M., Madigan, M. T., Swingley, W. D., Cheung, P. C., Clocksin, K. M., Conrad, A. L., ... Touchman, J. W. (2008). The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus. Journal of Bacteriology, 190(13), 4687-4696. https://doi.org/10.1128/JB.00299-08

The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus. / Sattley, W. Matthew; Madigan, Michael T.; Swingley, Wesley D.; Cheung, Patricia C.; Clocksin, Kate M.; Conrad, Amber L.; Dejesa, Liza C.; Honchak, Barbara M.; Jung, Deborah O.; Karbach, Lauren E.; Kurdoglu, Ahmet; Lahiri, Surobhi; Mastrian, Stephen D.; Page, Lawrence E.; Taylor, Heather L.; Wang, Zi T.; Raymond, Jason; Chen, Min; Blankenship, Robert E.; Touchman, Jeffrey W.

In: Journal of Bacteriology, Vol. 190, No. 13, 07.2008, p. 4687-4696.

Research output: Contribution to journalArticle

Sattley, WM, Madigan, MT, Swingley, WD, Cheung, PC, Clocksin, KM, Conrad, AL, Dejesa, LC, Honchak, BM, Jung, DO, Karbach, LE, Kurdoglu, A, Lahiri, S, Mastrian, SD, Page, LE, Taylor, HL, Wang, ZT, Raymond, J, Chen, M, Blankenship, RE & Touchman, JW 2008, 'The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus', Journal of Bacteriology, vol. 190, no. 13, pp. 4687-4696. https://doi.org/10.1128/JB.00299-08
Sattley, W. Matthew ; Madigan, Michael T. ; Swingley, Wesley D. ; Cheung, Patricia C. ; Clocksin, Kate M. ; Conrad, Amber L. ; Dejesa, Liza C. ; Honchak, Barbara M. ; Jung, Deborah O. ; Karbach, Lauren E. ; Kurdoglu, Ahmet ; Lahiri, Surobhi ; Mastrian, Stephen D. ; Page, Lawrence E. ; Taylor, Heather L. ; Wang, Zi T. ; Raymond, Jason ; Chen, Min ; Blankenship, Robert E. ; Touchman, Jeffrey W. / The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus. In: Journal of Bacteriology. 2008 ; Vol. 190, No. 13. pp. 4687-4696.
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