Abstract
Despite the fact that heliobacteria are the only phototrophic representatives of the bacterial phylum Firmicutes, genomic analyses of these organisms have yet to be reported. Here we describe the complete sequence and analysis of the genome of Heliobacterium modesticaldum, a thermophilic species belonging to this unique group of phototrophs. The genome is a single 3.1-Mb circular chromosome containing 3,138 open reading frames. As suspected from physiological studies of heliobacteria that have failed to show photoautotrophic growth, genes encoding enzymes for known autotrophic pathways in other phototrophic organisms, including ribulose bisphosphate carboxylase (Calvin cycle), citrate lyase (reverse citric acid cycle), and malyl coenzyme A lyase (3-hydroxypropionate pathway), are not present in the H. modesticaldum genome. Thus, heliobacteria appear to be the only known anaerobic anoxygenic phototrophs that are not capable of autotrophy. Although for some cellular activities, such as nitrogen fixation, there is a full complement of genes in H. modesticaldum, other processes, including carbon metabolism and endosporulation, are more genetically streamlined than they are in most other low-G+C gram-positive bacteria. Moreover, several genes encoding photosynthetic functions in phototrophic purple bacteria are not present in the heliobacteria. In contrast to the nutritional flexibility of many anoxygenic phototrophs, the complete genome sequence of H. modesticaldum reveals an organism with a notable degree of metabolic specialization and genomic reduction.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4687-4696 |
| Number of pages | 10 |
| Journal | Journal of Bacteriology |
| Volume | 190 |
| Issue number | 13 |
| DOIs | |
| State | Published - Jul 2008 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Immunology
Cite this
The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus. / Sattley, W. Matthew; Madigan, Michael T.; Swingley, Wesley D.; Cheung, Patricia C.; Clocksin, Kate M.; Conrad, Amber L.; Dejesa, Liza C.; Honchak, Barbara M.; Jung, Deborah O.; Karbach, Lauren E.; Kurdoglu, Ahmet; Lahiri, Surobhi; Mastrian, Stephen D.; Page, Lawrence E.; Taylor, Heather L.; Wang, Zi T.; Raymond, Jason; Chen, Min; Blankenship, Robert E.; Touchman, Jeffrey W.
In: Journal of Bacteriology, Vol. 190, No. 13, 07.2008, p. 4687-4696.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus
AU - Sattley, W. Matthew
AU - Madigan, Michael T.
AU - Swingley, Wesley D.
AU - Cheung, Patricia C.
AU - Clocksin, Kate M.
AU - Conrad, Amber L.
AU - Dejesa, Liza C.
AU - Honchak, Barbara M.
AU - Jung, Deborah O.
AU - Karbach, Lauren E.
AU - Kurdoglu, Ahmet
AU - Lahiri, Surobhi
AU - Mastrian, Stephen D.
AU - Page, Lawrence E.
AU - Taylor, Heather L.
AU - Wang, Zi T.
AU - Raymond, Jason
AU - Chen, Min
AU - Blankenship, Robert E.
AU - Touchman, Jeffrey W.
PY - 2008/7
Y1 - 2008/7
N2 - Despite the fact that heliobacteria are the only phototrophic representatives of the bacterial phylum Firmicutes, genomic analyses of these organisms have yet to be reported. Here we describe the complete sequence and analysis of the genome of Heliobacterium modesticaldum, a thermophilic species belonging to this unique group of phototrophs. The genome is a single 3.1-Mb circular chromosome containing 3,138 open reading frames. As suspected from physiological studies of heliobacteria that have failed to show photoautotrophic growth, genes encoding enzymes for known autotrophic pathways in other phototrophic organisms, including ribulose bisphosphate carboxylase (Calvin cycle), citrate lyase (reverse citric acid cycle), and malyl coenzyme A lyase (3-hydroxypropionate pathway), are not present in the H. modesticaldum genome. Thus, heliobacteria appear to be the only known anaerobic anoxygenic phototrophs that are not capable of autotrophy. Although for some cellular activities, such as nitrogen fixation, there is a full complement of genes in H. modesticaldum, other processes, including carbon metabolism and endosporulation, are more genetically streamlined than they are in most other low-G+C gram-positive bacteria. Moreover, several genes encoding photosynthetic functions in phototrophic purple bacteria are not present in the heliobacteria. In contrast to the nutritional flexibility of many anoxygenic phototrophs, the complete genome sequence of H. modesticaldum reveals an organism with a notable degree of metabolic specialization and genomic reduction.
AB - Despite the fact that heliobacteria are the only phototrophic representatives of the bacterial phylum Firmicutes, genomic analyses of these organisms have yet to be reported. Here we describe the complete sequence and analysis of the genome of Heliobacterium modesticaldum, a thermophilic species belonging to this unique group of phototrophs. The genome is a single 3.1-Mb circular chromosome containing 3,138 open reading frames. As suspected from physiological studies of heliobacteria that have failed to show photoautotrophic growth, genes encoding enzymes for known autotrophic pathways in other phototrophic organisms, including ribulose bisphosphate carboxylase (Calvin cycle), citrate lyase (reverse citric acid cycle), and malyl coenzyme A lyase (3-hydroxypropionate pathway), are not present in the H. modesticaldum genome. Thus, heliobacteria appear to be the only known anaerobic anoxygenic phototrophs that are not capable of autotrophy. Although for some cellular activities, such as nitrogen fixation, there is a full complement of genes in H. modesticaldum, other processes, including carbon metabolism and endosporulation, are more genetically streamlined than they are in most other low-G+C gram-positive bacteria. Moreover, several genes encoding photosynthetic functions in phototrophic purple bacteria are not present in the heliobacteria. In contrast to the nutritional flexibility of many anoxygenic phototrophs, the complete genome sequence of H. modesticaldum reveals an organism with a notable degree of metabolic specialization and genomic reduction.
UR - http://www.scopus.com/inward/record.url?scp=46049109918&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=46049109918&partnerID=8YFLogxK
U2 - 10.1128/JB.00299-08
DO - 10.1128/JB.00299-08
M3 - Article
C2 - 18441057
AN - SCOPUS:46049109918
VL - 190
SP - 4687
EP - 4696
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 13
ER -