The genetic basis of transgressive ovary size in honeybee workers

Timothy A. Linksvayer, Olav Rueppell, Adam Siegel, Osman Kaftanoglu, Robert Page, Gro Amdam

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Ovarioles are the functional unit of the female insect reproductive organs and the number of ovarioles per ovary strongly influences egg-laying rate and fecundity. Social evolution in the honeybee (Apis mellifera) has resulted in queens with 200-360 total ovarioles and workers with usually 20 or less. In addition, variation in ovariole number among workers relates to worker sensory tuning, foraging behavior, and the ability to lay unfertilized male-destined eggs. To study the genetic architecture of worker ovariole number, we performed a series of crosses between Africanized and European bees that differ in worker ovariole number. Unexpectedly, these crosses produced transgressive worker phenotypes with extreme ovariole numbers that were sensitive to the social environment. We used a new selective pooled DNA interval mapping approach with two Africanized backcrosses to identify quantitative trait loci (QTL) underlying the transgressive ovary phenotype. We identified one QTL on chromosome 11 and found some evidence for another QTL on chromosome 2. Both QTL regions contain plausible functional candidate genes. The ovariole number of foragers was correlated with the sugar concentration of collected nectar, supporting previous studies showing a link between worker physiology and foraging behavior. We discuss how the phenotype of extreme worker ovariole numbers and the underlying genetic factors we identified could be linked to the development of queen traits.

Original languageEnglish (US)
Pages (from-to)693-707
Number of pages15
JournalGenetics
Volume183
Issue number2
DOIs
StatePublished - 2009

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Quantitative Trait Loci
Ovary
Bees
Phenotype
Plant Nectar
Chromosomes, Human, Pair 11
Aptitude
Chromosomes, Human, Pair 2
Social Environment
Eggs
Ovum
Fertility
Insects
DNA
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Linksvayer, T. A., Rueppell, O., Siegel, A., Kaftanoglu, O., Page, R., & Amdam, G. (2009). The genetic basis of transgressive ovary size in honeybee workers. Genetics, 183(2), 693-707. https://doi.org/10.1534/genetics.109.105452

The genetic basis of transgressive ovary size in honeybee workers. / Linksvayer, Timothy A.; Rueppell, Olav; Siegel, Adam; Kaftanoglu, Osman; Page, Robert; Amdam, Gro.

In: Genetics, Vol. 183, No. 2, 2009, p. 693-707.

Research output: Contribution to journalArticle

Linksvayer, TA, Rueppell, O, Siegel, A, Kaftanoglu, O, Page, R & Amdam, G 2009, 'The genetic basis of transgressive ovary size in honeybee workers', Genetics, vol. 183, no. 2, pp. 693-707. https://doi.org/10.1534/genetics.109.105452
Linksvayer TA, Rueppell O, Siegel A, Kaftanoglu O, Page R, Amdam G. The genetic basis of transgressive ovary size in honeybee workers. Genetics. 2009;183(2):693-707. https://doi.org/10.1534/genetics.109.105452
Linksvayer, Timothy A. ; Rueppell, Olav ; Siegel, Adam ; Kaftanoglu, Osman ; Page, Robert ; Amdam, Gro. / The genetic basis of transgressive ovary size in honeybee workers. In: Genetics. 2009 ; Vol. 183, No. 2. pp. 693-707.
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