The gene structure of the Drosophila melanogaster homolog of the human proto-oncogene fos

Emma Rousseau, Elliott S. Goldstein

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The Drosophila melanogaster homolog of the human proto-oncogene fos is Dfos. It is the only fos homolog in the Drosophila genome. Fos functions as a subunit of the heterodimeric transcription factor AP-1. There are two models of the Dfos gene. The first comes from a cDNA sequence of Dfos (Perkins et al., Genes Dev. 4 (1990) 822). The second is from the gene sequence published by the Drosophila genome project (Adams et al., Science 287 (2000) 2185), and there are notable contradictions between the two models. The promoter and the 5′ end of the transcript sequence were not identified in either model. In this paper, we present the gene structure of Dfos and identify the promoter. This promoter has an initiator and a downstream promoter element sequence, but lacks a TATA box. Through comparison of the mRNA and genomic DNA sequences, three introns varying in length from 66 bp to 17.57 kb were found and verified by RT-PCR. The Dfos gene is 21.2 kb in length, giving a transcript of 3438 bp, coding for a predicted protein of 595 amino acids. The 3′ untranslated region is confirmed to be 1092 bp in length.

Original languageEnglish (US)
Pages (from-to)315-322
Number of pages8
JournalGene
Volume272
Issue number1-2
DOIs
StatePublished - Jul 11 2001

Fingerprint

Proto-Oncogenes
Drosophila melanogaster
Genes
Drosophila
Genome
TATA Box
Transcription Factor AP-1
3' Untranslated Regions
Introns
Complementary DNA
Amino Acids
Polymerase Chain Reaction
Messenger RNA
Proteins

Keywords

  • AP-1
  • Dfos
  • Djun
  • Transcription factor

ASJC Scopus subject areas

  • Genetics

Cite this

The gene structure of the Drosophila melanogaster homolog of the human proto-oncogene fos. / Rousseau, Emma; Goldstein, Elliott S.

In: Gene, Vol. 272, No. 1-2, 11.07.2001, p. 315-322.

Research output: Contribution to journalArticle

Rousseau, Emma ; Goldstein, Elliott S. / The gene structure of the Drosophila melanogaster homolog of the human proto-oncogene fos. In: Gene. 2001 ; Vol. 272, No. 1-2. pp. 315-322.
@article{b7c5f1981493468190725116adf0baea,
title = "The gene structure of the Drosophila melanogaster homolog of the human proto-oncogene fos",
abstract = "The Drosophila melanogaster homolog of the human proto-oncogene fos is Dfos. It is the only fos homolog in the Drosophila genome. Fos functions as a subunit of the heterodimeric transcription factor AP-1. There are two models of the Dfos gene. The first comes from a cDNA sequence of Dfos (Perkins et al., Genes Dev. 4 (1990) 822). The second is from the gene sequence published by the Drosophila genome project (Adams et al., Science 287 (2000) 2185), and there are notable contradictions between the two models. The promoter and the 5′ end of the transcript sequence were not identified in either model. In this paper, we present the gene structure of Dfos and identify the promoter. This promoter has an initiator and a downstream promoter element sequence, but lacks a TATA box. Through comparison of the mRNA and genomic DNA sequences, three introns varying in length from 66 bp to 17.57 kb were found and verified by RT-PCR. The Dfos gene is 21.2 kb in length, giving a transcript of 3438 bp, coding for a predicted protein of 595 amino acids. The 3′ untranslated region is confirmed to be 1092 bp in length.",
keywords = "AP-1, Dfos, Djun, Transcription factor",
author = "Emma Rousseau and Goldstein, {Elliott S.}",
year = "2001",
month = "7",
day = "11",
doi = "10.1016/S0378-1119(01)00514-5",
language = "English (US)",
volume = "272",
pages = "315--322",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - The gene structure of the Drosophila melanogaster homolog of the human proto-oncogene fos

AU - Rousseau, Emma

AU - Goldstein, Elliott S.

PY - 2001/7/11

Y1 - 2001/7/11

N2 - The Drosophila melanogaster homolog of the human proto-oncogene fos is Dfos. It is the only fos homolog in the Drosophila genome. Fos functions as a subunit of the heterodimeric transcription factor AP-1. There are two models of the Dfos gene. The first comes from a cDNA sequence of Dfos (Perkins et al., Genes Dev. 4 (1990) 822). The second is from the gene sequence published by the Drosophila genome project (Adams et al., Science 287 (2000) 2185), and there are notable contradictions between the two models. The promoter and the 5′ end of the transcript sequence were not identified in either model. In this paper, we present the gene structure of Dfos and identify the promoter. This promoter has an initiator and a downstream promoter element sequence, but lacks a TATA box. Through comparison of the mRNA and genomic DNA sequences, three introns varying in length from 66 bp to 17.57 kb were found and verified by RT-PCR. The Dfos gene is 21.2 kb in length, giving a transcript of 3438 bp, coding for a predicted protein of 595 amino acids. The 3′ untranslated region is confirmed to be 1092 bp in length.

AB - The Drosophila melanogaster homolog of the human proto-oncogene fos is Dfos. It is the only fos homolog in the Drosophila genome. Fos functions as a subunit of the heterodimeric transcription factor AP-1. There are two models of the Dfos gene. The first comes from a cDNA sequence of Dfos (Perkins et al., Genes Dev. 4 (1990) 822). The second is from the gene sequence published by the Drosophila genome project (Adams et al., Science 287 (2000) 2185), and there are notable contradictions between the two models. The promoter and the 5′ end of the transcript sequence were not identified in either model. In this paper, we present the gene structure of Dfos and identify the promoter. This promoter has an initiator and a downstream promoter element sequence, but lacks a TATA box. Through comparison of the mRNA and genomic DNA sequences, three introns varying in length from 66 bp to 17.57 kb were found and verified by RT-PCR. The Dfos gene is 21.2 kb in length, giving a transcript of 3438 bp, coding for a predicted protein of 595 amino acids. The 3′ untranslated region is confirmed to be 1092 bp in length.

KW - AP-1

KW - Dfos

KW - Djun

KW - Transcription factor

UR - http://www.scopus.com/inward/record.url?scp=0035845012&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035845012&partnerID=8YFLogxK

U2 - 10.1016/S0378-1119(01)00514-5

DO - 10.1016/S0378-1119(01)00514-5

M3 - Article

VL - 272

SP - 315

EP - 322

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-2

ER -