TY - JOUR
T1 - The fluorescent probe BCECF has a heterogenous distribution in sea urchin eggs
AU - Mozingo, Nancy M.
AU - Chandler, Douglas E.
N1 - Funding Information:
ACKNOWL.EDGEMENTS We would like to thank Charles Kazilek for preparing Figure 1 and for his excellenta ssistancew ith Figures 2-6. This study was supportedb y grants from the NSF (DCB-8810200)a nd NIH (HD-00619).
PY - 1990/8
Y1 - 1990/8
N2 - Sea urchin eggs were loaded with the pH-sensitive fluorescent probe BCECF. Homogenization of these eggs, followed by centrifugation, resulted in 25% of the total homogenate fluorescence remaining with the pellet. Fluorescence microscopy revealed brightly fluorescing punctate organelles whose fluorescence was not quenched by acidification. The excitation spectrum of intracellular BCECF was markedly red-shifted compared to probe calibrated in buffer. Model excitation spectra based on a two compartment model mimicked the intracellular BCECF spectrum, therefore, supporting the possibility that organelles in sea urchin eggs accumulate large amounts of BCECF in a relatively pH-insensitive form.
AB - Sea urchin eggs were loaded with the pH-sensitive fluorescent probe BCECF. Homogenization of these eggs, followed by centrifugation, resulted in 25% of the total homogenate fluorescence remaining with the pellet. Fluorescence microscopy revealed brightly fluorescing punctate organelles whose fluorescence was not quenched by acidification. The excitation spectrum of intracellular BCECF was markedly red-shifted compared to probe calibrated in buffer. Model excitation spectra based on a two compartment model mimicked the intracellular BCECF spectrum, therefore, supporting the possibility that organelles in sea urchin eggs accumulate large amounts of BCECF in a relatively pH-insensitive form.
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U2 - 10.1016/0309-1651(90)91188-A
DO - 10.1016/0309-1651(90)91188-A
M3 - Article
C2 - 2225083
AN - SCOPUS:0024992586
SN - 0309-1651
VL - 14
SP - 689
EP - 699
JO - Cell Biology International Reports
JF - Cell Biology International Reports
IS - 8
ER -