TY - JOUR
T1 - The FLEXGene repository
T2 - Exploiting the fruits of the genome projects by creating a needed resource to face the challenges of the post-genomic era
AU - Brizuela, Leonardo
AU - Richardson, Aaron
AU - Marsischky, Gerald
AU - Labaer, Joshua
N1 - Funding Information:
We would like to thank Ed Harlow for sharing his ideas and for providing continuous support and advice to the Institute. We would also like to thank Richard Kolodner for his support in the building of the yeast repository and all past and present members of the lab for their contributions and efforts. The Harvard Medical School, the Cystic Fibrosis Foundation, and the NIH are thanked for funding our work.
PY - 2002/7
Y1 - 2002/7
N2 - Thanks to the results of the multiple completed and ongoing genome sequencing projects and to the newly available recombination-based cloning techniques, it is now possible to build gene repositories with no precedent in their composition, formatting, and potential. This new type of gene repository is necessary to address the challenges imposed by the post-genomic era, i.e., experimentation on a genome-wide scale. We are building the FLEXGene (Full Length EXpression-ready) repository. This unique resource will contain clones representing the complete ORFeome of different organisms, including Homo sapiens as well as several pathogens and model organisms. It will consist of a comprehensive, characterized (sequence-verified), and arrayed gene repository. This resource will allow full exploitation of the genomic information by enabling genome-wide scale experimentation at the level of functional/phenotypic assays as well as at the level of protein expression, purification, and analysis. Here we describe the rationale and construction of this resource and focus on the data obtained from the Saccharomyces cerevisiae project.
AB - Thanks to the results of the multiple completed and ongoing genome sequencing projects and to the newly available recombination-based cloning techniques, it is now possible to build gene repositories with no precedent in their composition, formatting, and potential. This new type of gene repository is necessary to address the challenges imposed by the post-genomic era, i.e., experimentation on a genome-wide scale. We are building the FLEXGene (Full Length EXpression-ready) repository. This unique resource will contain clones representing the complete ORFeome of different organisms, including Homo sapiens as well as several pathogens and model organisms. It will consist of a comprehensive, characterized (sequence-verified), and arrayed gene repository. This resource will allow full exploitation of the genomic information by enabling genome-wide scale experimentation at the level of functional/phenotypic assays as well as at the level of protein expression, purification, and analysis. Here we describe the rationale and construction of this resource and focus on the data obtained from the Saccharomyces cerevisiae project.
KW - FLEXGene repository
KW - Proteomics
KW - Recombination cloning
KW - Yeast genome
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U2 - 10.1016/S0188-4409(02)00372-7
DO - 10.1016/S0188-4409(02)00372-7
M3 - Article
C2 - 12234520
AN - SCOPUS:0036662167
SN - 0188-4409
VL - 33
SP - 318
EP - 324
JO - Archives of Medical Research
JF - Archives of Medical Research
IS - 4
ER -