TY - JOUR
T1 - The fitness effects of a point mutation in Escherichia coli change with founding population density
AU - Cao, Huansheng
AU - Plague, Gordon R.
N1 - Funding Information:
We thank Kevin Dougherty and Evelyn Fetridge for insightful discussion, Yaping Yang for assistance in the lab, Dr. Yajun Yan for use of his lab for the growth curve measurements, and the anonymous reviewers for critically reviewing the manuscript. This study was funded by the National Institutes of Health (Grant Number 7R15GM081862-02 awarded to G.R.P.).
Publisher Copyright:
© 2016, Springer International Publishing Switzerland.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Although intraspecific competition plays a seminal role in organismal evolution, little is known about the fitness effects of mutations at different population densities. We identified a point mutation in the cyclic AMP receptor protein (CRP) gene in Escherichia coli that confers significantly higher fitness than the wildtype at low founding population density, but significantly lower fitness at high founding density. Because CRP is a transcription factor that regulates the expression of nearly 500 genes, we compared global gene expression profiles of the mutant and wildtype strains. This mutation (S63F) does not affect expression of crp itself, but it does significantly affect expression of 170 and 157 genes at high and low founding density, respectively. Interestingly, acid resistance genes, some of which are known to exhibit density-dependent effects in E. coli, were consistently differentially expressed at high but not low density. As such, these genes may play a key role in reducing the crp mutant’s fitness at high density, although other differentially expressed genes almost certainly also contribute to the fluctuating fitness differences we observed. Whatever the causes, we suspect that many mutations may exhibit density-dependent fitness effects in natural populations, so the fate of new mutations may frequently depend on the effective population size when they originate.
AB - Although intraspecific competition plays a seminal role in organismal evolution, little is known about the fitness effects of mutations at different population densities. We identified a point mutation in the cyclic AMP receptor protein (CRP) gene in Escherichia coli that confers significantly higher fitness than the wildtype at low founding population density, but significantly lower fitness at high founding density. Because CRP is a transcription factor that regulates the expression of nearly 500 genes, we compared global gene expression profiles of the mutant and wildtype strains. This mutation (S63F) does not affect expression of crp itself, but it does significantly affect expression of 170 and 157 genes at high and low founding density, respectively. Interestingly, acid resistance genes, some of which are known to exhibit density-dependent effects in E. coli, were consistently differentially expressed at high but not low density. As such, these genes may play a key role in reducing the crp mutant’s fitness at high density, although other differentially expressed genes almost certainly also contribute to the fluctuating fitness differences we observed. Whatever the causes, we suspect that many mutations may exhibit density-dependent fitness effects in natural populations, so the fate of new mutations may frequently depend on the effective population size when they originate.
KW - E. coli
KW - Effective population size
KW - Fluctuating fitness
KW - Natural selection
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U2 - 10.1007/s10709-016-9910-5
DO - 10.1007/s10709-016-9910-5
M3 - Article
C2 - 27344657
AN - SCOPUS:84976334383
VL - 144
SP - 417
EP - 424
JO - Genetica
JF - Genetica
SN - 0016-6707
IS - 4
ER -