The extraction of single-particle diffraction patterns from a multiple-particle diffraction pattern

A. V. Martin, A. J. Morgan, T. Ekeberg, N. D. Loh, F. R N C Maia, F. Wang, John Spence, H. N. Chapman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The structures of biological molecules may soon be determined with X-ray free-electron lasers without crystallization by recording the coherent diffraction patterns of many identical copies of a molecule. Most analysis methods require a measurement of each molecule individually. However, current injection methods deliver particles to the X-ray beam stochastically and the maximum yield of single particle measurements is 37% at optimal concentration. The remaining 63% of pulses intercept no particles or multiple particles. We demonstrate that in the latter case single particle diffraction patterns can be extracted provided the particles are sufficiently separated. The technique has the potential to greatly increase the amount of data available for three-dimensional imaging of identical particles with X-ray lasers.

Original languageEnglish (US)
Pages (from-to)15102-15112
Number of pages11
JournalOptics Express
Volume21
Issue number13
DOIs
StatePublished - Jul 1 2013

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diffraction patterns
molecules
x rays
free electron lasers
recording
crystallization
injection
pulses
lasers

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Martin, A. V., Morgan, A. J., Ekeberg, T., Loh, N. D., Maia, F. R. N. C., Wang, F., ... Chapman, H. N. (2013). The extraction of single-particle diffraction patterns from a multiple-particle diffraction pattern. Optics Express, 21(13), 15102-15112. https://doi.org/10.1364/OE.21.015102

The extraction of single-particle diffraction patterns from a multiple-particle diffraction pattern. / Martin, A. V.; Morgan, A. J.; Ekeberg, T.; Loh, N. D.; Maia, F. R N C; Wang, F.; Spence, John; Chapman, H. N.

In: Optics Express, Vol. 21, No. 13, 01.07.2013, p. 15102-15112.

Research output: Contribution to journalArticle

Martin, AV, Morgan, AJ, Ekeberg, T, Loh, ND, Maia, FRNC, Wang, F, Spence, J & Chapman, HN 2013, 'The extraction of single-particle diffraction patterns from a multiple-particle diffraction pattern', Optics Express, vol. 21, no. 13, pp. 15102-15112. https://doi.org/10.1364/OE.21.015102
Martin AV, Morgan AJ, Ekeberg T, Loh ND, Maia FRNC, Wang F et al. The extraction of single-particle diffraction patterns from a multiple-particle diffraction pattern. Optics Express. 2013 Jul 1;21(13):15102-15112. https://doi.org/10.1364/OE.21.015102
Martin, A. V. ; Morgan, A. J. ; Ekeberg, T. ; Loh, N. D. ; Maia, F. R N C ; Wang, F. ; Spence, John ; Chapman, H. N. / The extraction of single-particle diffraction patterns from a multiple-particle diffraction pattern. In: Optics Express. 2013 ; Vol. 21, No. 13. pp. 15102-15112.
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