The extraction of single-particle diffraction patterns from a multiple-particle diffraction pattern

A. V. Martin, A. J. Morgan, T. Ekeberg, N. D. Loh, F. R N C Maia, F. Wang, John Spence, H. N. Chapman

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The structures of biological molecules may soon be determined with X-ray free-electron lasers without crystallization by recording the coherent diffraction patterns of many identical copies of a molecule. Most analysis methods require a measurement of each molecule individually. However, current injection methods deliver particles to the X-ray beam stochastically and the maximum yield of single particle measurements is 37% at optimal concentration. The remaining 63% of pulses intercept no particles or multiple particles. We demonstrate that in the latter case single particle diffraction patterns can be extracted provided the particles are sufficiently separated. The technique has the potential to greatly increase the amount of data available for three-dimensional imaging of identical particles with X-ray lasers.

Original languageEnglish (US)
Pages (from-to)15102-15112
Number of pages11
JournalOptics Express
Volume21
Issue number13
DOIs
StatePublished - Jul 1 2013

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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    Martin, A. V., Morgan, A. J., Ekeberg, T., Loh, N. D., Maia, F. R. N. C., Wang, F., Spence, J., & Chapman, H. N. (2013). The extraction of single-particle diffraction patterns from a multiple-particle diffraction pattern. Optics Express, 21(13), 15102-15112. https://doi.org/10.1364/OE.21.015102