The expression and genomic organization of randomly selected cloned Drosophila melanogaster genes

Elliott S. Goldstein, Walter S. Vincent, Kathleen A. Schultz

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A lambda recombinant DNA library containing Drosophila melanogaster nuclear DNA inserts was screened with cDNA made from oocyte and gastrula poly(A)+ RNA. 124 clones were isolated which represented sequences complementary to a distribution of abundancies of their RNAs. The clone set was then used as probes to identify those whose RNA abundancies changed during embryonic development. The vast majority of clones showed little difference during development. Four different clones were identified whose poly(A)+ RNAs were quantitatively regulated; two were oocyte-specific, and two were embryonic-specific. 44 clones were chosen for in situ hybridization to salivary gland polytene chromosomes. The location and distribution of their sites are described. A class of clones, identified by in situ hybridization to the nucleolus, is further described. These clones contain a scrambled array of ribosomal intervening sequences.

Original languageEnglish (US)
Pages (from-to)209-219
Number of pages11
JournalBBA - Gene Structure and Expression
Volume867
Issue number4
DOIs
StatePublished - Aug 22 1986

Fingerprint

Drosophila melanogaster
Clone Cells
Genes
RNA
Messenger RNA
Recombinant DNA
Chromosomes
Gene Library
Introns
Complementary DNA
DNA
Oocytes
In Situ Hybridization
Polytene Chromosomes
Gastrula
Salivary Glands
Embryonic Development

Keywords

  • (D melanogaster)
  • Development
  • Embyrogenesis
  • In situ hybridization
  • mRNA population

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

The expression and genomic organization of randomly selected cloned Drosophila melanogaster genes. / Goldstein, Elliott S.; Vincent, Walter S.; Schultz, Kathleen A.

In: BBA - Gene Structure and Expression, Vol. 867, No. 4, 22.08.1986, p. 209-219.

Research output: Contribution to journalArticle

Goldstein, Elliott S. ; Vincent, Walter S. ; Schultz, Kathleen A. / The expression and genomic organization of randomly selected cloned Drosophila melanogaster genes. In: BBA - Gene Structure and Expression. 1986 ; Vol. 867, No. 4. pp. 209-219.
@article{86d0dde287094af0926ff6fac2c283a4,
title = "The expression and genomic organization of randomly selected cloned Drosophila melanogaster genes",
abstract = "A lambda recombinant DNA library containing Drosophila melanogaster nuclear DNA inserts was screened with cDNA made from oocyte and gastrula poly(A)+ RNA. 124 clones were isolated which represented sequences complementary to a distribution of abundancies of their RNAs. The clone set was then used as probes to identify those whose RNA abundancies changed during embryonic development. The vast majority of clones showed little difference during development. Four different clones were identified whose poly(A)+ RNAs were quantitatively regulated; two were oocyte-specific, and two were embryonic-specific. 44 clones were chosen for in situ hybridization to salivary gland polytene chromosomes. The location and distribution of their sites are described. A class of clones, identified by in situ hybridization to the nucleolus, is further described. These clones contain a scrambled array of ribosomal intervening sequences.",
keywords = "(D melanogaster), Development, Embyrogenesis, In situ hybridization, mRNA population",
author = "Goldstein, {Elliott S.} and Vincent, {Walter S.} and Schultz, {Kathleen A.}",
year = "1986",
month = "8",
day = "22",
doi = "10.1016/0167-4781(86)90036-9",
language = "English (US)",
volume = "867",
pages = "209--219",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "4",

}

TY - JOUR

T1 - The expression and genomic organization of randomly selected cloned Drosophila melanogaster genes

AU - Goldstein, Elliott S.

AU - Vincent, Walter S.

AU - Schultz, Kathleen A.

PY - 1986/8/22

Y1 - 1986/8/22

N2 - A lambda recombinant DNA library containing Drosophila melanogaster nuclear DNA inserts was screened with cDNA made from oocyte and gastrula poly(A)+ RNA. 124 clones were isolated which represented sequences complementary to a distribution of abundancies of their RNAs. The clone set was then used as probes to identify those whose RNA abundancies changed during embryonic development. The vast majority of clones showed little difference during development. Four different clones were identified whose poly(A)+ RNAs were quantitatively regulated; two were oocyte-specific, and two were embryonic-specific. 44 clones were chosen for in situ hybridization to salivary gland polytene chromosomes. The location and distribution of their sites are described. A class of clones, identified by in situ hybridization to the nucleolus, is further described. These clones contain a scrambled array of ribosomal intervening sequences.

AB - A lambda recombinant DNA library containing Drosophila melanogaster nuclear DNA inserts was screened with cDNA made from oocyte and gastrula poly(A)+ RNA. 124 clones were isolated which represented sequences complementary to a distribution of abundancies of their RNAs. The clone set was then used as probes to identify those whose RNA abundancies changed during embryonic development. The vast majority of clones showed little difference during development. Four different clones were identified whose poly(A)+ RNAs were quantitatively regulated; two were oocyte-specific, and two were embryonic-specific. 44 clones were chosen for in situ hybridization to salivary gland polytene chromosomes. The location and distribution of their sites are described. A class of clones, identified by in situ hybridization to the nucleolus, is further described. These clones contain a scrambled array of ribosomal intervening sequences.

KW - (D melanogaster)

KW - Development

KW - Embyrogenesis

KW - In situ hybridization

KW - mRNA population

UR - http://www.scopus.com/inward/record.url?scp=0022545461&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022545461&partnerID=8YFLogxK

U2 - 10.1016/0167-4781(86)90036-9

DO - 10.1016/0167-4781(86)90036-9

M3 - Article

C2 - 3017431

AN - SCOPUS:0022545461

VL - 867

SP - 209

EP - 219

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 4

ER -