The expression and genomic organization of randomly selected cloned Drosophila melanogaster genes

Elliott S. Goldstein, Walter S. Vincent, Kathleen A. Schultz

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A lambda recombinant DNA library containing Drosophila melanogaster nuclear DNA inserts was screened with cDNA made from oocyte and gastrula poly(A)+ RNA. 124 clones were isolated which represented sequences complementary to a distribution of abundancies of their RNAs. The clone set was then used as probes to identify those whose RNA abundancies changed during embryonic development. The vast majority of clones showed little difference during development. Four different clones were identified whose poly(A)+ RNAs were quantitatively regulated; two were oocyte-specific, and two were embryonic-specific. 44 clones were chosen for in situ hybridization to salivary gland polytene chromosomes. The location and distribution of their sites are described. A class of clones, identified by in situ hybridization to the nucleolus, is further described. These clones contain a scrambled array of ribosomal intervening sequences.

Original languageEnglish (US)
Pages (from-to)209-219
Number of pages11
JournalBBA - Gene Structure and Expression
Volume867
Issue number4
DOIs
StatePublished - Aug 22 1986

Keywords

  • (D melanogaster)
  • Development
  • Embyrogenesis
  • In situ hybridization
  • mRNA population

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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