The Elg1 clamp loader plays a role in sister chromatid cohesion

Oren Parnas, Adi Zipin-Roitman, Yuval Mazor, Batia Liefshitz, Shay Ben-Aroya, Martin Kupiec

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Mutations in the ELG1 gene of yeast lead to genomic instability, manifested in high levels of genetic recombination, chromosome loss, and gross chromosomal rearrangements. Elg1 shows similarity to the large subunit of the Replication Factor C clamp loader, and forms a RFC-like (RLC) complex in conjunction with the 4 small RFC subunits. Two additional RLCs exist in yeast: in one of them the large subunit is Ctf18, and in the other, Rad24. Ctf18 has been characterized as the RLC that functions in sister chromatid cohesion. Here we present evidence that the Elg1 RLC (but not Rad24) also plays an important role in this process. A genetic screen identified the cohesin subunit Mcd1/Scc1 and its loader Scc2 as suppressors of the synthetic lethality between elg1 and ctf4. We describe genetic interactions between ELG1 and genes encoding cohesin subunits and their accessory proteins. We also show that defects in Elg1 lead to higher precocious sister chromatid separation, and that Ctf18 and Elg1 affect cohesion via a joint pathway. Finally, we localize both Ctf18 and Elg1 to chromatin and show that Elg1 plays a role in the recruitment of Ctf18. Our results suggest that Elg1, Ctf4, and Ctf18 may coordinate the relative movement of the replication fork with respect to the cohesin ring.

Original languageEnglish (US)
Article numbere5497
JournalPLoS One
Volume4
Issue number5
DOIs
StatePublished - May 11 2009
Externally publishedYes

Fingerprint

loaders
Loaders
Chromatids
chromatids
Clamping devices
cohesion
yeasts
chromosome elimination
genetic recombination
Yeast
Replication Protein C
Yeasts
chromatin
Gene encoding
genes
Genomic Instability
Accessories
Chromosomes
mutation
genomics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Parnas, O., Zipin-Roitman, A., Mazor, Y., Liefshitz, B., Ben-Aroya, S., & Kupiec, M. (2009). The Elg1 clamp loader plays a role in sister chromatid cohesion. PLoS One, 4(5), [e5497]. https://doi.org/10.1371/journal.pone.0005497

The Elg1 clamp loader plays a role in sister chromatid cohesion. / Parnas, Oren; Zipin-Roitman, Adi; Mazor, Yuval; Liefshitz, Batia; Ben-Aroya, Shay; Kupiec, Martin.

In: PLoS One, Vol. 4, No. 5, e5497, 11.05.2009.

Research output: Contribution to journalArticle

Parnas, O, Zipin-Roitman, A, Mazor, Y, Liefshitz, B, Ben-Aroya, S & Kupiec, M 2009, 'The Elg1 clamp loader plays a role in sister chromatid cohesion', PLoS One, vol. 4, no. 5, e5497. https://doi.org/10.1371/journal.pone.0005497
Parnas O, Zipin-Roitman A, Mazor Y, Liefshitz B, Ben-Aroya S, Kupiec M. The Elg1 clamp loader plays a role in sister chromatid cohesion. PLoS One. 2009 May 11;4(5). e5497. https://doi.org/10.1371/journal.pone.0005497
Parnas, Oren ; Zipin-Roitman, Adi ; Mazor, Yuval ; Liefshitz, Batia ; Ben-Aroya, Shay ; Kupiec, Martin. / The Elg1 clamp loader plays a role in sister chromatid cohesion. In: PLoS One. 2009 ; Vol. 4, No. 5.
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