The electrochemical behavior of a FAD dependent glucose dehydrogenase with direct electron transfer subunit by immobilization on self-assembled monolayers

Inyoung Lee, Noya Loew, Wakako Tsugawa, Chi En Lin, David Probst, Jeffrey LaBelle, Koji Sode

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Continuous glucose monitoring (CGM) is a vital technology for diabetes patients by providing tight glycemic control. Currently, many commercially available CGM sensors use glucose oxidase (GOD) as sensor element, but this enzyme is not able to transfer electrons directly to the electrode without oxygen or an electronic mediator. We previously reported a mutated FAD dependent glucose dehydrogenase complex (FADGDH) capable of direct electron transfer (DET) via an electron transfer subunit without involving oxygen or a mediator. In this study, we investigated the electrochemical response of DET by controlling the immobilization of DET-FADGDH using 3 types of self-assembled monolayers (SAMs) with varying lengths. With the employment of DET-FADGDH and SAM, high current densities were achieved without being affected by interfering substances such as acetaminophen and ascorbic acid. Additionally, the current generated from DET-FADGDH electrodes decreased with increasing length of SAM, suggesting that the DET ability can be affected by the distance between the enzyme and the electrode. These results indicate the feasibility of controlling the immobilization state of the enzymes on the electrode surface.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalBioelectrochemistry
Volume121
DOIs
StatePublished - Jun 1 2018

Fingerprint

Glucose 1-Dehydrogenase
Flavin-Adenine Dinucleotide
dehydrogenases
Self assembled monolayers
immobilization
glucose
Immobilization
Glucose
electron transfer
Electrons
enzymes
Electrodes
electrodes
Enzymes
Biosensing Techniques
Oxygen
Glucose Oxidase
Glucose oxidase
Oxidoreductases
Monitoring

Keywords

  • Direct electron transfer
  • FADGDH
  • Glucose sensor
  • Self-assembled monolayer

ASJC Scopus subject areas

  • Biophysics
  • Physical and Theoretical Chemistry
  • Electrochemistry

Cite this

The electrochemical behavior of a FAD dependent glucose dehydrogenase with direct electron transfer subunit by immobilization on self-assembled monolayers. / Lee, Inyoung; Loew, Noya; Tsugawa, Wakako; Lin, Chi En; Probst, David; LaBelle, Jeffrey; Sode, Koji.

In: Bioelectrochemistry, Vol. 121, 01.06.2018, p. 1-6.

Research output: Contribution to journalArticle

Lee, Inyoung ; Loew, Noya ; Tsugawa, Wakako ; Lin, Chi En ; Probst, David ; LaBelle, Jeffrey ; Sode, Koji. / The electrochemical behavior of a FAD dependent glucose dehydrogenase with direct electron transfer subunit by immobilization on self-assembled monolayers. In: Bioelectrochemistry. 2018 ; Vol. 121. pp. 1-6.
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AU - Probst, David

AU - LaBelle, Jeffrey

AU - Sode, Koji

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