Abstract
Batch kinetic experiments were carried out to measure conjugative-transfer kinetics for plasmid RP4, which encodes resistance to kanamycin, tetracycline and ampicillin. The donor species was Rhodobacter capsulatus, while the recipient was a Pseudomonas strain obtained in our laboratory. Evaluated was a mass-action kinetic model that had a donor-to-recipient rate coefficient k11 and a transconjugant-to-recipient rate coefficient k2. Experimental results demonstrated that k11 and k12 were of similar magnitudes and could be reasonably large: e.g. up to 0.03 l gT/gD gR day for k11. However, the rate coefficients were not constants. Instead, they increased dramatically as the energy availability increased. Energy was made available in kinetic experiments from the energy substrates and from internal storage materials, carried over from prior batch growth cultures.
Original language | English (US) |
---|---|
Pages (from-to) | 461-468 |
Number of pages | 8 |
Journal | Water Research |
Volume | 26 |
Issue number | 4 |
DOIs | |
State | Published - Apr 1992 |
Externally published | Yes |
Keywords
- biological process
- conjugation
- energy availability
- kinetics
- modeling
- plasmid transfer
ASJC Scopus subject areas
- Environmental Engineering
- Civil and Structural Engineering
- Ecological Modeling
- Water Science and Technology
- Waste Management and Disposal
- Pollution