The effects of energy availability on the conjugative-transfer kinetics of plasmid RP4

Jacqueline A. MacDonald, Barth F. Smets, Bruce E. Rittmann

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Batch kinetic experiments were carried out to measure conjugative-transfer kinetics for plasmid RP4, which encodes resistance to kanamycin, tetracycline and ampicillin. The donor species was Rhodobacter capsulatus, while the recipient was a Pseudomonas strain obtained in our laboratory. Evaluated was a mass-action kinetic model that had a donor-to-recipient rate coefficient k11 and a transconjugant-to-recipient rate coefficient k2. Experimental results demonstrated that k11 and k12 were of similar magnitudes and could be reasonably large: e.g. up to 0.03 l gT/gD gR day for k11. However, the rate coefficients were not constants. Instead, they increased dramatically as the energy availability increased. Energy was made available in kinetic experiments from the energy substrates and from internal storage materials, carried over from prior batch growth cultures.

Original languageEnglish (US)
Pages (from-to)461-468
Number of pages8
JournalWater Research
Volume26
Issue number4
DOIs
StatePublished - Apr 1992
Externally publishedYes

Keywords

  • biological process
  • conjugation
  • energy availability
  • kinetics
  • modeling
  • plasmid transfer

ASJC Scopus subject areas

  • Ecological Modeling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

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