The effect of local variation in malaria transmission on the prevalence of sulfadoxine-pyrimethamine resistant haplotypes and selective sweep characteristics in Malawi

Elena Artimovich, Atupele Kapito-Tembo, Paul Pensulo, Osward Nyirenda, Sarah Brown, Sudhaunshu Joshi, Terrie E. Taylor, Don Mathanga, Ananias A. Escalante, Miriam K. Laufer, Shannon Takala-Harrison

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Persistence of sulfadoxine-pyrimethamine (SP) resistance has been described in an urban setting in Malawi where malaria transmission is relatively low. Higher malaria transmission is associated with greater genetic diversity and more frequent genetic recombination, which could lead to a more rapid re-emergence of SP-sensitive parasites, as well as more rapid degradation of selective sweeps. In this study, the impact of local variation in malaria transmission on the prevalence of SP-resistant haplotypes and selective sweep characteristics was investigated at an urban site with low parasite prevalence and two rural sites with moderate and high parasite prevalence. Methods: Samples from three sites with different parasite prevalence were genotyped for resistance markers within pfdhfr-ts and pfdhps and at microsatellites flanking these genes. Expected heterozygosity (He) was estimated to evaluate genetic diversity. Results: No difference in the prevalence of highly resistant DHFR 51I/59R/108N and DHPS 437G/540E was found between sites. Small differences in He flanking pfdhfr-ts and pfdhps were seen between rural-moderate and the other sites, as well as some shared haplotypes between the rural-high and urban-low sites. Conclusions: The results do not show an effect of local variation in malaria transmission, as inferred from parasite prevalence, on SP-resistant haplotype prevalence.

Original languageEnglish (US)
Article number860
JournalMalaria Journal
Volume14
Issue number1
DOIs
StatePublished - Oct 5 2015
Externally publishedYes

Fingerprint

Malawi
Haplotypes
Malaria
Parasites
pyrimethamine drug combination fanasil
Microsatellite Repeats
Genetic Recombination
Genes

Keywords

  • Dihydrofolate reductase (DHFR) and dihydropteroate synthase (DHPS)
  • Malaria
  • Resistance
  • Selective sweeps
  • Sulfadoxine-pyrimethamine

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology

Cite this

The effect of local variation in malaria transmission on the prevalence of sulfadoxine-pyrimethamine resistant haplotypes and selective sweep characteristics in Malawi. / Artimovich, Elena; Kapito-Tembo, Atupele; Pensulo, Paul; Nyirenda, Osward; Brown, Sarah; Joshi, Sudhaunshu; Taylor, Terrie E.; Mathanga, Don; Escalante, Ananias A.; Laufer, Miriam K.; Takala-Harrison, Shannon.

In: Malaria Journal, Vol. 14, No. 1, 860, 05.10.2015.

Research output: Contribution to journalArticle

Artimovich, E, Kapito-Tembo, A, Pensulo, P, Nyirenda, O, Brown, S, Joshi, S, Taylor, TE, Mathanga, D, Escalante, AA, Laufer, MK & Takala-Harrison, S 2015, 'The effect of local variation in malaria transmission on the prevalence of sulfadoxine-pyrimethamine resistant haplotypes and selective sweep characteristics in Malawi', Malaria Journal, vol. 14, no. 1, 860. https://doi.org/10.1186/s12936-015-0860-7
Artimovich, Elena ; Kapito-Tembo, Atupele ; Pensulo, Paul ; Nyirenda, Osward ; Brown, Sarah ; Joshi, Sudhaunshu ; Taylor, Terrie E. ; Mathanga, Don ; Escalante, Ananias A. ; Laufer, Miriam K. ; Takala-Harrison, Shannon. / The effect of local variation in malaria transmission on the prevalence of sulfadoxine-pyrimethamine resistant haplotypes and selective sweep characteristics in Malawi. In: Malaria Journal. 2015 ; Vol. 14, No. 1.
@article{93e05f1f563c4d97a6b755b22892b74a,
title = "The effect of local variation in malaria transmission on the prevalence of sulfadoxine-pyrimethamine resistant haplotypes and selective sweep characteristics in Malawi",
abstract = "Background: Persistence of sulfadoxine-pyrimethamine (SP) resistance has been described in an urban setting in Malawi where malaria transmission is relatively low. Higher malaria transmission is associated with greater genetic diversity and more frequent genetic recombination, which could lead to a more rapid re-emergence of SP-sensitive parasites, as well as more rapid degradation of selective sweeps. In this study, the impact of local variation in malaria transmission on the prevalence of SP-resistant haplotypes and selective sweep characteristics was investigated at an urban site with low parasite prevalence and two rural sites with moderate and high parasite prevalence. Methods: Samples from three sites with different parasite prevalence were genotyped for resistance markers within pfdhfr-ts and pfdhps and at microsatellites flanking these genes. Expected heterozygosity (He) was estimated to evaluate genetic diversity. Results: No difference in the prevalence of highly resistant DHFR 51I/59R/108N and DHPS 437G/540E was found between sites. Small differences in He flanking pfdhfr-ts and pfdhps were seen between rural-moderate and the other sites, as well as some shared haplotypes between the rural-high and urban-low sites. Conclusions: The results do not show an effect of local variation in malaria transmission, as inferred from parasite prevalence, on SP-resistant haplotype prevalence.",
keywords = "Dihydrofolate reductase (DHFR) and dihydropteroate synthase (DHPS), Malaria, Resistance, Selective sweeps, Sulfadoxine-pyrimethamine",
author = "Elena Artimovich and Atupele Kapito-Tembo and Paul Pensulo and Osward Nyirenda and Sarah Brown and Sudhaunshu Joshi and Taylor, {Terrie E.} and Don Mathanga and Escalante, {Ananias A.} and Laufer, {Miriam K.} and Shannon Takala-Harrison",
year = "2015",
month = "10",
day = "5",
doi = "10.1186/s12936-015-0860-7",
language = "English (US)",
volume = "14",
journal = "Malaria Journal",
issn = "1475-2875",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - The effect of local variation in malaria transmission on the prevalence of sulfadoxine-pyrimethamine resistant haplotypes and selective sweep characteristics in Malawi

AU - Artimovich, Elena

AU - Kapito-Tembo, Atupele

AU - Pensulo, Paul

AU - Nyirenda, Osward

AU - Brown, Sarah

AU - Joshi, Sudhaunshu

AU - Taylor, Terrie E.

AU - Mathanga, Don

AU - Escalante, Ananias A.

AU - Laufer, Miriam K.

AU - Takala-Harrison, Shannon

PY - 2015/10/5

Y1 - 2015/10/5

N2 - Background: Persistence of sulfadoxine-pyrimethamine (SP) resistance has been described in an urban setting in Malawi where malaria transmission is relatively low. Higher malaria transmission is associated with greater genetic diversity and more frequent genetic recombination, which could lead to a more rapid re-emergence of SP-sensitive parasites, as well as more rapid degradation of selective sweeps. In this study, the impact of local variation in malaria transmission on the prevalence of SP-resistant haplotypes and selective sweep characteristics was investigated at an urban site with low parasite prevalence and two rural sites with moderate and high parasite prevalence. Methods: Samples from three sites with different parasite prevalence were genotyped for resistance markers within pfdhfr-ts and pfdhps and at microsatellites flanking these genes. Expected heterozygosity (He) was estimated to evaluate genetic diversity. Results: No difference in the prevalence of highly resistant DHFR 51I/59R/108N and DHPS 437G/540E was found between sites. Small differences in He flanking pfdhfr-ts and pfdhps were seen between rural-moderate and the other sites, as well as some shared haplotypes between the rural-high and urban-low sites. Conclusions: The results do not show an effect of local variation in malaria transmission, as inferred from parasite prevalence, on SP-resistant haplotype prevalence.

AB - Background: Persistence of sulfadoxine-pyrimethamine (SP) resistance has been described in an urban setting in Malawi where malaria transmission is relatively low. Higher malaria transmission is associated with greater genetic diversity and more frequent genetic recombination, which could lead to a more rapid re-emergence of SP-sensitive parasites, as well as more rapid degradation of selective sweeps. In this study, the impact of local variation in malaria transmission on the prevalence of SP-resistant haplotypes and selective sweep characteristics was investigated at an urban site with low parasite prevalence and two rural sites with moderate and high parasite prevalence. Methods: Samples from three sites with different parasite prevalence were genotyped for resistance markers within pfdhfr-ts and pfdhps and at microsatellites flanking these genes. Expected heterozygosity (He) was estimated to evaluate genetic diversity. Results: No difference in the prevalence of highly resistant DHFR 51I/59R/108N and DHPS 437G/540E was found between sites. Small differences in He flanking pfdhfr-ts and pfdhps were seen between rural-moderate and the other sites, as well as some shared haplotypes between the rural-high and urban-low sites. Conclusions: The results do not show an effect of local variation in malaria transmission, as inferred from parasite prevalence, on SP-resistant haplotype prevalence.

KW - Dihydrofolate reductase (DHFR) and dihydropteroate synthase (DHPS)

KW - Malaria

KW - Resistance

KW - Selective sweeps

KW - Sulfadoxine-pyrimethamine

UR - http://www.scopus.com/inward/record.url?scp=84946471604&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84946471604&partnerID=8YFLogxK

U2 - 10.1186/s12936-015-0860-7

DO - 10.1186/s12936-015-0860-7

M3 - Article

AN - SCOPUS:84946471604

VL - 14

JO - Malaria Journal

JF - Malaria Journal

SN - 1475-2875

IS - 1

M1 - 860

ER -