TY - JOUR
T1 - The effect of fetal calf serum on growth arrest caused by activators of protein kinase C
AU - Bradshaw, Tracey D.
AU - Gescher, Andreas
AU - Pettit, George R.
PY - 1991/4/1
Y1 - 1991/4/1
N2 - The growth of human‐derived A549 lung carcinoma cells is inhibited by activators of protein kinase C (PKC) such as 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA). In this study, the effect of serum deprivation on TPA‐induced growth retardation has been investigated. Cells cultured with 10% FCS and TPA (10−8 M) stopped growing for 6 days, whereas inhibition of DNA synthesis caused by TPA in cells which were grown in medium containing the serum substitute ultraser lasted for less than 48 hr. The ability of cells to respond to the growth‐inhibitory potential of TPA decreased with decreasing amounts of FCS in the cellular medium. Addition of fetuin or epidermal growth factor (EGF) to incubates with serum‐deprived cells increased the ability of TPA to affect growth, but addition of platelet‐derived growth factor (PDGF), transforming growth factor beta (TGF‐β) or retinoic acid (RA) was without effect. Growth arrest caused by bryostatin I, another PKC activator, was equally transitory in serum‐supplemented and serum‐deprived cells. Cytosol of serum‐deprived cells contained only 32% of specific phorbol ester binding sites compared to cells grown with FCS; PKC enzyme activity and immunodectable protein were similarly reduced in cells grown without FCS. There was no difference in rate of TPA‐induced down‐regulation of PKC activity and cytosolic phorbol ester receptor sites between cells grown with or without serum.
AB - The growth of human‐derived A549 lung carcinoma cells is inhibited by activators of protein kinase C (PKC) such as 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA). In this study, the effect of serum deprivation on TPA‐induced growth retardation has been investigated. Cells cultured with 10% FCS and TPA (10−8 M) stopped growing for 6 days, whereas inhibition of DNA synthesis caused by TPA in cells which were grown in medium containing the serum substitute ultraser lasted for less than 48 hr. The ability of cells to respond to the growth‐inhibitory potential of TPA decreased with decreasing amounts of FCS in the cellular medium. Addition of fetuin or epidermal growth factor (EGF) to incubates with serum‐deprived cells increased the ability of TPA to affect growth, but addition of platelet‐derived growth factor (PDGF), transforming growth factor beta (TGF‐β) or retinoic acid (RA) was without effect. Growth arrest caused by bryostatin I, another PKC activator, was equally transitory in serum‐supplemented and serum‐deprived cells. Cytosol of serum‐deprived cells contained only 32% of specific phorbol ester binding sites compared to cells grown with FCS; PKC enzyme activity and immunodectable protein were similarly reduced in cells grown without FCS. There was no difference in rate of TPA‐induced down‐regulation of PKC activity and cytosolic phorbol ester receptor sites between cells grown with or without serum.
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U2 - 10.1002/ijc.2910470624
DO - 10.1002/ijc.2910470624
M3 - Article
C2 - 1707036
AN - SCOPUS:0025774641
SN - 0020-7136
VL - 47
SP - 929
EP - 932
JO - International Journal of Cancer
JF - International Journal of Cancer
IS - 6
ER -