The effect of developmental stage on the sensitivity of cell and body size to hypoxia in Drosophila melanogaster

Erica C. Heinrich, Manoush Farzin, C. Jaco Klok, Jon Harrison

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Animals reared in hypoxic environments frequently exhibit smaller body sizes than when reared under normal atmospheric oxygen concentrations. The mechanisms responsible for this widely documented pattern of body size plasticity are poorly known. We studied the ontogeny of responses of Drosophila melanogaster adult body size to hypoxic exposure. We hypothesized that there may be critical oxygen-sensitive periods during D. melanogaster development that are primarily responsive to body size regulation. Instead, our results showed that exposure to hypoxia (an atmospheric partial pressure of oxygen of 10?kPa) during any developmental stage (embryo, larvae and pupae) leads to smaller adult size. However, short hypoxic exposures during the late larval and early pupal stages had the greatest effects on adult size. We then investigated whether the observed reductions in size induced by hypoxia at various developmental stages were the result of a decrease in cell size or cell number. Abdominal epithelial cells of flies reared continuously in hypoxia were smaller in mean diameter and were size-limited compared with cells of flies reared in normoxia. Flies reared in hypoxia during the embryonic, larval or pupal stage, or during their entire development, had smaller wing areas than flies reared in normoxia. Flies reared during the pupal stage, or throughout development in hypoxia had smaller wing cells, even after controlling for the effect of wing size. These results suggest that hypoxia effects on the body size of D. melanogaster probably occur by multiple mechanisms operating at various developmental stages.

Original languageEnglish (US)
Pages (from-to)1419-1427
Number of pages9
JournalJournal of Experimental Biology
Volume214
Issue number9
DOIs
StatePublished - May 2011

Fingerprint

Body Size
hypoxia
Drosophila melanogaster
Cell Size
developmental stage
body size
Diptera
developmental stages
normoxia
cells
Oxygen
oxygen
Pupa
Atmospheric Pressure
Partial Pressure
pupa
partial pressure
ontogeny
atmospheric pressure
Larva

Keywords

  • Development
  • Oxygen
  • Size

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Physiology
  • Insect Science
  • Aquatic Science

Cite this

The effect of developmental stage on the sensitivity of cell and body size to hypoxia in Drosophila melanogaster. / Heinrich, Erica C.; Farzin, Manoush; Klok, C. Jaco; Harrison, Jon.

In: Journal of Experimental Biology, Vol. 214, No. 9, 05.2011, p. 1419-1427.

Research output: Contribution to journalArticle

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