The Effect of Bacteriochlorophyll g Oxidation on Energy and Electron Transfer in Reaction Centers from Heliobacterium modesticaldum

Bryan Ferlez, Weibing Dong, Reza Siavashi, Kevin Redding, Harvey J M Hou, John H. Golbeck, Art Van Der Est

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The heliobacteria are a family of strictly anaerobic, Gram-positive, photoheterotrophs in the Firmicutes. They make use of a homodimeric type I reaction center (RC) that contains ~20 antenna bacteriochlorophyll (BChl) g molecules, a special pair of BChl g′ molecules (P800), two 81-OH-Chl aF molecules (A0), a [4Fe-4S] iron-sulfur cluster (FX), and a carotenoid (4,4′-diaponeurosporene). It is known that in the presence of light and oxygen BChl g is converted to a species with an absorption spectrum identical to that of Chl a. Here, we show that main product of the conversion is 81-OH-Chl aF. Smaller amounts of two other oxidized Chl aF species are also produced. In the presence of light and oxygen, the kinetics of the conversion are monophasic and temperature dependent, with an activation energy of 66 ± 2 kJ mol-1. In the presence of oxygen in the dark, the conversion occurs in two temperature-dependent kinetic phases: a slow phase followed by a fast phase with an activation energy of 53 ± 1 kJ mol-1. The loss of BChl g′ occurs at the same rate as the loss of Bchl g; hence, the special pair converts at the same rate as the antenna Chls. However, the loss of P800 photooxidiation and flavodoxin reduction is not linear with the loss of BChl g. In anaerobic RCs, the charge recombination between P800 + and FX - at 80 K is monophasic with a lifetime of 4.2 ms, but after exposure to oxygen, an additional phase with a lifetime of 0.3 ms is observed. Transient EPR data show that the line width of P800 + increases as BChl g is converted to Chl aF and the rate of electron transfer from A0 to FX, as estimated from the net polarization generated by singlet-triplet mixing during the lifetime of P800 +A0 -, is unchanged. The transient EPR data also show that conversion of the BChl g results in increased formation of triplet states of both BChl g and Chl aF. The nonlinear loss of P800 photooxidiation and flavodoxin reduction, the biphasic backreaction kinetics, and the increased EPR line width of P800 + are all consistent with a model in which the BChl g′/BChl g′ and BChl g′/Chl aF′ special pairs are functional but the Chl aF′/Chl aF′ special pair is not.

Original languageEnglish (US)
Pages (from-to)13714-13725
Number of pages12
JournalJournal of Physical Chemistry B
Volume119
Issue number43
DOIs
StatePublished - Oct 29 2015

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Bacteriochlorophylls
electron transfer
energy transfer
Oxidation
oxidation
Electrons
Paramagnetic resonance
Oxygen
oxygen
Linewidth
life (durability)
Molecules
Kinetics
kinetics
antennas
Activation energy
Antennas
activation energy
Flavodoxin
molecules

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Materials Chemistry
  • Surfaces, Coatings and Films

Cite this

The Effect of Bacteriochlorophyll g Oxidation on Energy and Electron Transfer in Reaction Centers from Heliobacterium modesticaldum. / Ferlez, Bryan; Dong, Weibing; Siavashi, Reza; Redding, Kevin; Hou, Harvey J M; Golbeck, John H.; Van Der Est, Art.

In: Journal of Physical Chemistry B, Vol. 119, No. 43, 29.10.2015, p. 13714-13725.

Research output: Contribution to journalArticle

Ferlez, Bryan ; Dong, Weibing ; Siavashi, Reza ; Redding, Kevin ; Hou, Harvey J M ; Golbeck, John H. ; Van Der Est, Art. / The Effect of Bacteriochlorophyll g Oxidation on Energy and Electron Transfer in Reaction Centers from Heliobacterium modesticaldum. In: Journal of Physical Chemistry B. 2015 ; Vol. 119, No. 43. pp. 13714-13725.
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