The effect of a single, temperature-sensitive mutation on global gene expression in Escherichia coli

Yong Li, Kyle Cole, Sidney Altman

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

High-density DNA microarrays have been used to explore the genomic profiling of gene expression of a defective Escherichia coli strain with a temperature-sensitive mutation in the protein component of RNase P. A novel gene cluster was discovered in which two of the genes are known substrates of RNase P. The expression pattern of essential genes and gene discovery from intergenic regions, for which other new transcripts are found, are also discussed.

Original languageEnglish (US)
Pages (from-to)518-532
Number of pages15
JournalRNA
Volume9
Issue number5
DOIs
StatePublished - May 1 2003
Externally publishedYes

Fingerprint

Ribonuclease P
Escherichia coli
Gene Expression
Mutation
Intergenic DNA
Temperature
Essential Genes
Genetic Association Studies
Gene Expression Profiling
Multigene Family
Oligonucleotide Array Sequence Analysis
Genes
Proteins

Keywords

  • Intergenic RNAs
  • RNase E
  • RNase P
  • Substrates

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

The effect of a single, temperature-sensitive mutation on global gene expression in Escherichia coli. / Li, Yong; Cole, Kyle; Altman, Sidney.

In: RNA, Vol. 9, No. 5, 01.05.2003, p. 518-532.

Research output: Contribution to journalArticle

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