The detection, isolation and characterization of a light-harvesting complex which is specifically associated with Photosystem I

Philip Haworth, Janet L. Watson, Charles J. Arntzen

Research output: Contribution to journalArticle

159 Scopus citations

Abstract

10% of the chlorophyll associated with a 'native' Photosystem (PS) I complex (110 chlorophylls/P-700) is chlorophyll (Chl) b. The Chl b is associated with a specific PS I antenna complex which we designate as LHC-I (i.e., a light-harvesting complex serving PS I). When the native PS I complex is degraded to the core complex by LHC-I extraction, there is a parallel loss of Chl b, fluorescence at 735 nm, together with 647 and 686 nm circular dichroism spectral properties, as well as a group of polypeptides of 24-19 kDa. In this paper we present a method by which the LHC-I complex can be dissociated from the native PS I. The isolated LHC-I contains significant amounts of Chl b (Chl a b {reversed tilde equals} 3.7). The long-wavelength fluorescence at 730 nm and circular dichroism signal at 686 nm observed in native PS I are maintained in this isolated complex. This isolated fraction also contains the low molecular weight polypeptides lost in the preparation of PS I core complex. We conclude that we have isolated the PS I antenna in an intact state and discuss its in vivo function.

Original languageEnglish (US)
Pages (from-to)151-158
Number of pages8
JournalBBA - Bioenergetics
Volume724
Issue number1
DOIs
StatePublished - Jul 29 1983

Keywords

  • (Pea chloroplast)
  • Chlorophyll b
  • Light - harvesting complex
  • Photosynthesis
  • Photosystem I

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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