The dangers of 'splicing and dicing': on the use of chimeric transcriptional activators in vitro

Thomas Kodadek; Stephen A. Johnston

doi:10.1016/1074-5521(95)90268-6

The dangers of 'splicing and dicing': on the use of chimeric transcriptional activators in vitro

Research output: Contribution to journal › Comment/debate › peer-review

Abstract

Chimeric transcription factors composed of heterologous DNA-binding and activation domains are often used to study the regulation of gene expression. The fact that such preparations also contain molecules in which only one of the two domains is functional is often overlooked, but a surprisingly small proportion of inactive domains could cause serious problems in the interpretation of quantitative data.

Original language	English (US)
Pages (from-to)	187-194
Number of pages	8
Journal	Chemistry and Biology
Volume	2
Issue number	4
DOIs	https://doi.org/10.1016/1074-5521(95)90268-6
State	Published - Apr 1995
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

Access to Document

10.1016/1074-5521(95)90268-6

Cite this

@article{7ce54903e2c843b49687e6ce3187a201,

title = "The dangers of 'splicing and dicing': on the use of chimeric transcriptional activators in vitro",

abstract = "Chimeric transcription factors composed of heterologous DNA-binding and activation domains are often used to study the regulation of gene expression. The fact that such preparations also contain molecules in which only one of the two domains is functional is often overlooked, but a surprisingly small proportion of inactive domains could cause serious problems in the interpretation of quantitative data.",

author = "Thomas Kodadek and Johnston, {Stephen A.}",

year = "1995",

month = apr,

doi = "10.1016/1074-5521(95)90268-6",

language = "English (US)",

volume = "2",

pages = "187--194",

journal = "Cell Chemical Biology",

issn = "2451-9448",

publisher = "Elsevier Inc.",

number = "4",

}

TY - JOUR

T1 - The dangers of 'splicing and dicing'

T2 - on the use of chimeric transcriptional activators in vitro

AU - Kodadek, Thomas

AU - Johnston, Stephen A.

PY - 1995/4

Y1 - 1995/4

N2 - Chimeric transcription factors composed of heterologous DNA-binding and activation domains are often used to study the regulation of gene expression. The fact that such preparations also contain molecules in which only one of the two domains is functional is often overlooked, but a surprisingly small proportion of inactive domains could cause serious problems in the interpretation of quantitative data.

AB - Chimeric transcription factors composed of heterologous DNA-binding and activation domains are often used to study the regulation of gene expression. The fact that such preparations also contain molecules in which only one of the two domains is functional is often overlooked, but a surprisingly small proportion of inactive domains could cause serious problems in the interpretation of quantitative data.

UR - http://www.scopus.com/inward/record.url?scp=0029278672&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029278672&partnerID=8YFLogxK

U2 - 10.1016/1074-5521(95)90268-6

DO - 10.1016/1074-5521(95)90268-6

M3 - Comment/debate

C2 - 9383420

AN - SCOPUS:0029278672

SN - 2451-9448

VL - 2

SP - 187

EP - 194

JO - Cell Chemical Biology

JF - Cell Chemical Biology

IS - 4

ER -

The dangers of 'splicing and dicing': on the use of chimeric transcriptional activators in vitro

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this