The dangers of 'splicing and dicing': on the use of chimeric transcriptional activators in vitro

Thomas Kodadek, Stephen A. Johnston

Research output: Contribution to journalComment/debate

2 Scopus citations

Abstract

Chimeric transcription factors composed of heterologous DNA-binding and activation domains are often used to study the regulation of gene expression. The fact that such preparations also contain molecules in which only one of the two domains is functional is often overlooked, but a surprisingly small proportion of inactive domains could cause serious problems in the interpretation of quantitative data.

Original languageEnglish (US)
Pages (from-to)187-194
Number of pages8
JournalChemistry and Biology
Volume2
Issue number4
DOIs
StatePublished - Apr 1995
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'The dangers of 'splicing and dicing': on the use of chimeric transcriptional activators in vitro'. Together they form a unique fingerprint.

  • Cite this