TY - JOUR
T1 - The dangers of 'splicing and dicing'
T2 - on the use of chimeric transcriptional activators in vitro
AU - Kodadek, Thomas
AU - Johnston, Stephen A.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1995/4
Y1 - 1995/4
N2 - Chimeric transcription factors composed of heterologous DNA-binding and activation domains are often used to study the regulation of gene expression. The fact that such preparations also contain molecules in which only one of the two domains is functional is often overlooked, but a surprisingly small proportion of inactive domains could cause serious problems in the interpretation of quantitative data.
AB - Chimeric transcription factors composed of heterologous DNA-binding and activation domains are often used to study the regulation of gene expression. The fact that such preparations also contain molecules in which only one of the two domains is functional is often overlooked, but a surprisingly small proportion of inactive domains could cause serious problems in the interpretation of quantitative data.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029278672&partnerID=8YFLogxK
U2 - 10.1016/1074-5521(95)90268-6
DO - 10.1016/1074-5521(95)90268-6
M3 - Comment/debate
C2 - 9383420
AN - SCOPUS:0029278672
VL - 2
SP - 187
EP - 194
JO - Cell Chemical Biology
JF - Cell Chemical Biology
SN - 2451-9448
IS - 4
ER -