The dangers of 'splicing and dicing': on the use of chimeric transcriptional activators in vitro

Thomas Kodadek; Stephen A. Johnston

doi:10.1016/1074-5521(95)90268-6

The dangers of 'splicing and dicing': on the use of chimeric transcriptional activators in vitro

Thomas Kodadek, Stephen A. Johnston

CLAS-NS: Life Sciences, School of (SOLS)

Research output: Contribution to journal › Comment/debate › peer-review

2 Scopus citations

Abstract

Chimeric transcription factors composed of heterologous DNA-binding and activation domains are often used to study the regulation of gene expression. The fact that such preparations also contain molecules in which only one of the two domains is functional is often overlooked, but a surprisingly small proportion of inactive domains could cause serious problems in the interpretation of quantitative data.

Original language	English (US)
Pages (from-to)	187-194
Number of pages	8
Journal	Chemistry and Biology
Volume	2
Issue number	4
DOIs	https://doi.org/10.1016/1074-5521(95)90268-6
State	Published - Apr 1995

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

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abstract = "Chimeric transcription factors composed of heterologous DNA-binding and activation domains are often used to study the regulation of gene expression. The fact that such preparations also contain molecules in which only one of the two domains is functional is often overlooked, but a surprisingly small proportion of inactive domains could cause serious problems in the interpretation of quantitative data.",

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