The cytoskeletal organization of breast carcinoma and fibroblast cells inside three dimensional (3-D) isotropic silicon microstructures

Mehdi Nikkhah, Jeannine S. Strobl, Raffaella De Vita, Masoud Agah

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Studying the cytoskeletal organization as cells interact in their local microenvironment is interest of biological science, tissue engineering and cancer diagnosis applications. Herein, we describe the behavior of cell lines obtained from metastatic breast tumor pleural effusions (MDA-MB-231), normal fibrocystic mammary epithelium (MCF10A), and HS68 normal fibroblasts inside three dimensional (3-D) isotropic silicon microstructures fabricated by a single-mask, single-isotropic-etch process. We report differences in adhesion, mechanism of force balance within the cytoskeleton, and deformability among these cell types inside the 3-D microenvironment. HS68 fibroblasts typically stretched and formed vinculin-rich focal adhesions at anchor sites inside the etched cavities. In contrast, MCF10A and MDA-MB-231 cells adopted the curved surfaces of isotropic microstructures and exhibited more diffuse vinculin cytoplasmic staining in addition to vinculin localized in focal adhesions. The measurement of cells elasticity using atomic force microscopy (AFM) indentation revealed that HS68 cells are significantly stiffer (p < 0.0001) than MCF10A and MDA-MB-231 cells. Upon microtubule disruption with nocodazole, fibroblasts no longer stretched, but adhesion of MCF10A and MDA-MB-231 within the etched features remained unaltered. Our findings are consistent with tensegrity theory. The 3-D microstructures have the potential to probe cytoskeletal-based differences between healthy and diseased cells that can provide biomarkers for diagnostics purposes.

Original languageEnglish (US)
Pages (from-to)4552-4561
Number of pages10
JournalBiomaterials
Volume31
Issue number16
DOIs
StatePublished - Jun 2010
Externally publishedYes

Fingerprint

Silicon
Fibroblasts
Vinculin
Adhesion
Cells
Breast Neoplasms
Microstructure
Focal Adhesions
Nocodazole
Biomarkers
Formability
Anchors
Tissue engineering
Indentation
Tumors
Masks
Elasticity
Atomic force microscopy
Biological Science Disciplines
Atomic Force Microscopy

Keywords

  • AFM
  • Breast cancer
  • HS68 fibroblasts
  • Isotropic
  • MEMS
  • Silicon

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

The cytoskeletal organization of breast carcinoma and fibroblast cells inside three dimensional (3-D) isotropic silicon microstructures. / Nikkhah, Mehdi; Strobl, Jeannine S.; De Vita, Raffaella; Agah, Masoud.

In: Biomaterials, Vol. 31, No. 16, 06.2010, p. 4552-4561.

Research output: Contribution to journalArticle

Nikkhah, Mehdi ; Strobl, Jeannine S. ; De Vita, Raffaella ; Agah, Masoud. / The cytoskeletal organization of breast carcinoma and fibroblast cells inside three dimensional (3-D) isotropic silicon microstructures. In: Biomaterials. 2010 ; Vol. 31, No. 16. pp. 4552-4561.
@article{8212b86beb2c48e0b85809ee811c333e,
title = "The cytoskeletal organization of breast carcinoma and fibroblast cells inside three dimensional (3-D) isotropic silicon microstructures",
abstract = "Studying the cytoskeletal organization as cells interact in their local microenvironment is interest of biological science, tissue engineering and cancer diagnosis applications. Herein, we describe the behavior of cell lines obtained from metastatic breast tumor pleural effusions (MDA-MB-231), normal fibrocystic mammary epithelium (MCF10A), and HS68 normal fibroblasts inside three dimensional (3-D) isotropic silicon microstructures fabricated by a single-mask, single-isotropic-etch process. We report differences in adhesion, mechanism of force balance within the cytoskeleton, and deformability among these cell types inside the 3-D microenvironment. HS68 fibroblasts typically stretched and formed vinculin-rich focal adhesions at anchor sites inside the etched cavities. In contrast, MCF10A and MDA-MB-231 cells adopted the curved surfaces of isotropic microstructures and exhibited more diffuse vinculin cytoplasmic staining in addition to vinculin localized in focal adhesions. The measurement of cells elasticity using atomic force microscopy (AFM) indentation revealed that HS68 cells are significantly stiffer (p < 0.0001) than MCF10A and MDA-MB-231 cells. Upon microtubule disruption with nocodazole, fibroblasts no longer stretched, but adhesion of MCF10A and MDA-MB-231 within the etched features remained unaltered. Our findings are consistent with tensegrity theory. The 3-D microstructures have the potential to probe cytoskeletal-based differences between healthy and diseased cells that can provide biomarkers for diagnostics purposes.",
keywords = "AFM, Breast cancer, HS68 fibroblasts, Isotropic, MEMS, Silicon",
author = "Mehdi Nikkhah and Strobl, {Jeannine S.} and {De Vita}, Raffaella and Masoud Agah",
year = "2010",
month = "6",
doi = "10.1016/j.biomaterials.2010.02.034",
language = "English (US)",
volume = "31",
pages = "4552--4561",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "16",

}

TY - JOUR

T1 - The cytoskeletal organization of breast carcinoma and fibroblast cells inside three dimensional (3-D) isotropic silicon microstructures

AU - Nikkhah, Mehdi

AU - Strobl, Jeannine S.

AU - De Vita, Raffaella

AU - Agah, Masoud

PY - 2010/6

Y1 - 2010/6

N2 - Studying the cytoskeletal organization as cells interact in their local microenvironment is interest of biological science, tissue engineering and cancer diagnosis applications. Herein, we describe the behavior of cell lines obtained from metastatic breast tumor pleural effusions (MDA-MB-231), normal fibrocystic mammary epithelium (MCF10A), and HS68 normal fibroblasts inside three dimensional (3-D) isotropic silicon microstructures fabricated by a single-mask, single-isotropic-etch process. We report differences in adhesion, mechanism of force balance within the cytoskeleton, and deformability among these cell types inside the 3-D microenvironment. HS68 fibroblasts typically stretched and formed vinculin-rich focal adhesions at anchor sites inside the etched cavities. In contrast, MCF10A and MDA-MB-231 cells adopted the curved surfaces of isotropic microstructures and exhibited more diffuse vinculin cytoplasmic staining in addition to vinculin localized in focal adhesions. The measurement of cells elasticity using atomic force microscopy (AFM) indentation revealed that HS68 cells are significantly stiffer (p < 0.0001) than MCF10A and MDA-MB-231 cells. Upon microtubule disruption with nocodazole, fibroblasts no longer stretched, but adhesion of MCF10A and MDA-MB-231 within the etched features remained unaltered. Our findings are consistent with tensegrity theory. The 3-D microstructures have the potential to probe cytoskeletal-based differences between healthy and diseased cells that can provide biomarkers for diagnostics purposes.

AB - Studying the cytoskeletal organization as cells interact in their local microenvironment is interest of biological science, tissue engineering and cancer diagnosis applications. Herein, we describe the behavior of cell lines obtained from metastatic breast tumor pleural effusions (MDA-MB-231), normal fibrocystic mammary epithelium (MCF10A), and HS68 normal fibroblasts inside three dimensional (3-D) isotropic silicon microstructures fabricated by a single-mask, single-isotropic-etch process. We report differences in adhesion, mechanism of force balance within the cytoskeleton, and deformability among these cell types inside the 3-D microenvironment. HS68 fibroblasts typically stretched and formed vinculin-rich focal adhesions at anchor sites inside the etched cavities. In contrast, MCF10A and MDA-MB-231 cells adopted the curved surfaces of isotropic microstructures and exhibited more diffuse vinculin cytoplasmic staining in addition to vinculin localized in focal adhesions. The measurement of cells elasticity using atomic force microscopy (AFM) indentation revealed that HS68 cells are significantly stiffer (p < 0.0001) than MCF10A and MDA-MB-231 cells. Upon microtubule disruption with nocodazole, fibroblasts no longer stretched, but adhesion of MCF10A and MDA-MB-231 within the etched features remained unaltered. Our findings are consistent with tensegrity theory. The 3-D microstructures have the potential to probe cytoskeletal-based differences between healthy and diseased cells that can provide biomarkers for diagnostics purposes.

KW - AFM

KW - Breast cancer

KW - HS68 fibroblasts

KW - Isotropic

KW - MEMS

KW - Silicon

UR - http://www.scopus.com/inward/record.url?scp=77950060016&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950060016&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2010.02.034

DO - 10.1016/j.biomaterials.2010.02.034

M3 - Article

C2 - 20207413

AN - SCOPUS:77950060016

VL - 31

SP - 4552

EP - 4561

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 16

ER -