The cytoplasmic organization of hyphal tip cells in the fungus Allomyces macrogynus

Maria M. Vargas, J. M. Aronson, Robert Roberson

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Light and transmission electron microscopy were used to examine hyphal tip cells of the fungus Allomyces macrogynus (Chytridiomycetes). A well defined apical body, i.e., Spitzenkörper, was observed at the extreme apex of hyphal cells. This distinctive, spherical cytoplasmic region consisted of a granular matrix devoid of ribosomes and most organelles. To our knowledge this is the first report describing such a structure in hyphae of an aseptate fungus. Vesicles (45-65 nm diameter) were concentrated in the peripheral cytoplasm of the apex, while relatively few were observed within the Spitzenkörper. Filasomes, spherical patches of dense fibrillar material containing a microvesicle core, were abundant in the apical regions near the plasma membrane. Microtubules traversed the Spitzenkörper at various angles and were in close association with the plasma membrane. Microfilaments were observed as individual elements in the cytoplasm or were organized into bundles. Individual microfilaments were frequently in close association with the plasma membrane, vesicles and microtubules. In the immediate subapical region mitochondria, multivesicular bodies, microbodies, Golgi equivalents and nuclei were abundant.

Original languageEnglish (US)
Pages (from-to)43-52
Number of pages10
JournalProtoplasma
Volume176
Issue number1-2
DOIs
StatePublished - Mar 1993

Fingerprint

Allomyces
Fungi
plasma membrane
Cell Membrane
microfilaments
Actin Cytoskeleton
Microtubules
microtubules
fungi
Cytoplasm
cytoplasm
Chytridiomycetes
Microbodies
Multivesicular Bodies
microbodies
Hyphae
cells
ribosomes
Transmission Electron Microscopy
Ribosomes

Keywords

  • Cryofixation
  • Cytoskeleton
  • Freeze substitution
  • Hyphal tip growth
  • Light microscopy
  • Spitzenkörper
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Cell Biology
  • Plant Science

Cite this

The cytoplasmic organization of hyphal tip cells in the fungus Allomyces macrogynus. / Vargas, Maria M.; Aronson, J. M.; Roberson, Robert.

In: Protoplasma, Vol. 176, No. 1-2, 03.1993, p. 43-52.

Research output: Contribution to journalArticle

Vargas, Maria M. ; Aronson, J. M. ; Roberson, Robert. / The cytoplasmic organization of hyphal tip cells in the fungus Allomyces macrogynus. In: Protoplasma. 1993 ; Vol. 176, No. 1-2. pp. 43-52.
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