The cytoplasmic organization of hyphal tip cells in the fungus Allomyces macrogynus

Maria M. Vargas, J. M. Aronson, Robert Roberson

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Light and transmission electron microscopy were used to examine hyphal tip cells of the fungus Allomyces macrogynus (Chytridiomycetes). A well defined apical body, i.e., Spitzenkörper, was observed at the extreme apex of hyphal cells. This distinctive, spherical cytoplasmic region consisted of a granular matrix devoid of ribosomes and most organelles. To our knowledge this is the first report describing such a structure in hyphae of an aseptate fungus. Vesicles (45-65 nm diameter) were concentrated in the peripheral cytoplasm of the apex, while relatively few were observed within the Spitzenkörper. Filasomes, spherical patches of dense fibrillar material containing a microvesicle core, were abundant in the apical regions near the plasma membrane. Microtubules traversed the Spitzenkörper at various angles and were in close association with the plasma membrane. Microfilaments were observed as individual elements in the cytoplasm or were organized into bundles. Individual microfilaments were frequently in close association with the plasma membrane, vesicles and microtubules. In the immediate subapical region mitochondria, multivesicular bodies, microbodies, Golgi equivalents and nuclei were abundant.

Original languageEnglish (US)
Pages (from-to)43-52
Number of pages10
JournalProtoplasma
Volume176
Issue number1-2
DOIs
StatePublished - Mar 1 1993

Keywords

  • Cryofixation
  • Cytoskeleton
  • Freeze substitution
  • Hyphal tip growth
  • Light microscopy
  • Spitzenkörper
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'The cytoplasmic organization of hyphal tip cells in the fungus Allomyces macrogynus'. Together they form a unique fingerprint.

  • Cite this