The cytoplasmic dot staining pattern is detected in a subgroup of patients with primary biliary cirrhosis

Donald B. Bloch, Hong Yu Jiang, Wei Hong Yang, Fiona Graeme-Cook, Keith Lindor, Anjali Viswanathan, Kenneth D. Bloch, Ayako Nakajima

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective. To determine the clinical significance of the cytoplasmic dot anti-"nuclear" antibody (ANA) staining pattern. Methods. We describe a patient with fatigue, arthralgias, elevated serum transaminase, and antibodies staining 5-20 cytoplasmic dots in HEp-2 cells. A liver biopsy revealed the presence of Stage III primary biliary cirrhosis (PBC). Using 2-color immunofluorescence, we determined the relationship between the cytoplasmic dot staining pattern and that produced by antibodies directed against the GW182 component of mRNA processing bodies. To determine the prevalence of the cytoplasmic dot staining pattern in patients with PBC, sera from 493 patients were tested for antibodies producing this staining pattern. Results. Antibodies in our patient's serum colocalized with anti-GW182 antibodies in cytoplasmic dots, but did not react with recombinant GW182, suggesting that they were directed against an additional component(s) of these structures. The cytoplasmic dot staining pattern was observed in 21 of 493 (4.3%) patients with PBC. In comparison, this staining pattern was not produced by serum from 248 patients with other autoimmune diseases. Conclusion. A subset of patients with PBC have autoantibodies that produce the cytoplasmic dot staining pattern. These antibodies react with one or more as yet unidentified components of the mRNA processing body. Appreciation of the clinical significance of the cytoplasmic dot staining pattern may assist in appropriate diagnosis and treatment of patients with PBC.

Original languageEnglish (US)
Pages (from-to)477-483
Number of pages7
JournalJournal of Rheumatology
Volume32
Issue number3
StatePublished - Mar 2005
Externally publishedYes

Fingerprint

Biliary Liver Cirrhosis
Staining and Labeling
Antibodies
Serum
Anti-Idiotypic Antibodies
Messenger RNA
Arthralgia
Transaminases
Autoantibodies
Autoimmune Diseases
Fatigue
Fluorescent Antibody Technique
Color
Biopsy
Liver

Keywords

  • Antinuclear antibodies
  • Cytoplasmic autoantigens
  • Primary biliary cirrhosis

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Bloch, D. B., Jiang, H. Y., Yang, W. H., Graeme-Cook, F., Lindor, K., Viswanathan, A., ... Nakajima, A. (2005). The cytoplasmic dot staining pattern is detected in a subgroup of patients with primary biliary cirrhosis. Journal of Rheumatology, 32(3), 477-483.

The cytoplasmic dot staining pattern is detected in a subgroup of patients with primary biliary cirrhosis. / Bloch, Donald B.; Jiang, Hong Yu; Yang, Wei Hong; Graeme-Cook, Fiona; Lindor, Keith; Viswanathan, Anjali; Bloch, Kenneth D.; Nakajima, Ayako.

In: Journal of Rheumatology, Vol. 32, No. 3, 03.2005, p. 477-483.

Research output: Contribution to journalArticle

Bloch, DB, Jiang, HY, Yang, WH, Graeme-Cook, F, Lindor, K, Viswanathan, A, Bloch, KD & Nakajima, A 2005, 'The cytoplasmic dot staining pattern is detected in a subgroup of patients with primary biliary cirrhosis', Journal of Rheumatology, vol. 32, no. 3, pp. 477-483.
Bloch DB, Jiang HY, Yang WH, Graeme-Cook F, Lindor K, Viswanathan A et al. The cytoplasmic dot staining pattern is detected in a subgroup of patients with primary biliary cirrhosis. Journal of Rheumatology. 2005 Mar;32(3):477-483.
Bloch, Donald B. ; Jiang, Hong Yu ; Yang, Wei Hong ; Graeme-Cook, Fiona ; Lindor, Keith ; Viswanathan, Anjali ; Bloch, Kenneth D. ; Nakajima, Ayako. / The cytoplasmic dot staining pattern is detected in a subgroup of patients with primary biliary cirrhosis. In: Journal of Rheumatology. 2005 ; Vol. 32, No. 3. pp. 477-483.
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abstract = "Objective. To determine the clinical significance of the cytoplasmic dot anti-{"}nuclear{"} antibody (ANA) staining pattern. Methods. We describe a patient with fatigue, arthralgias, elevated serum transaminase, and antibodies staining 5-20 cytoplasmic dots in HEp-2 cells. A liver biopsy revealed the presence of Stage III primary biliary cirrhosis (PBC). Using 2-color immunofluorescence, we determined the relationship between the cytoplasmic dot staining pattern and that produced by antibodies directed against the GW182 component of mRNA processing bodies. To determine the prevalence of the cytoplasmic dot staining pattern in patients with PBC, sera from 493 patients were tested for antibodies producing this staining pattern. Results. Antibodies in our patient's serum colocalized with anti-GW182 antibodies in cytoplasmic dots, but did not react with recombinant GW182, suggesting that they were directed against an additional component(s) of these structures. The cytoplasmic dot staining pattern was observed in 21 of 493 (4.3{\%}) patients with PBC. In comparison, this staining pattern was not produced by serum from 248 patients with other autoimmune diseases. Conclusion. A subset of patients with PBC have autoantibodies that produce the cytoplasmic dot staining pattern. These antibodies react with one or more as yet unidentified components of the mRNA processing body. Appreciation of the clinical significance of the cytoplasmic dot staining pattern may assist in appropriate diagnosis and treatment of patients with PBC.",
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