The cysteine-peptidase bleomycin hydrolase is a member of the galactose regulon in yeast

Wenjin Zheng, H. Eric Xu, Stephen Johnston

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Bleomycin hydrolase is a cysteine peptidase discovered through its ability to detoxify the anti-cancer glycopeptide, bleomycin. Although found in all tissues in mammals and in both eukaryotes and prokaryotes, the normal cellular function of this peptidase is not known. We had previously reported the purification of bleomycin hydrolase from yeast based on its unexpected ability to bind DNA. Recently we collaborated in solving the crystal structure of this protein, revealing a hexameric ring organization. We now report that the molecular characterization of the gene encoding yeast bleomycin hydrolase is also surprising. The transcription of the gene is regulated by galactose. Furthermore, this regulation is conveyed by a binding site for the Gal4 regulatory protein in its promotor, prompting the designation of this gene as GAL6. Gal6p also appears to have a negative effect on the GAL system as a deletion of the gene leads to a 2-5-fold higher expression of the GAL1, GAL2, GAL7, and MEL1 genes. The GAL6 deletion does not affect the expression of another inducible gene, HSP26. Neither the peptidase nor the nucleic acid binding activity of Gal6p as assayed is apparently required to convey this regulation, implying yet another function for this new member of the GAL regulon.

Original languageEnglish (US)
Pages (from-to)30350-30355
Number of pages6
JournalJournal of Biological Chemistry
Volume272
Issue number48
DOIs
StatePublished - Nov 28 1997
Externally publishedYes

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Regulon
Galactose
Yeast
Cysteine
Peptide Hydrolases
Genes
Yeasts
Gene encoding
Mammals
Glycopeptides
Bleomycin
Gene Deletion
Transcription
Eukaryota
Nucleic Acids
Purification
Proteins
Crystal structure
Binding Sites
bleomycin hydrolase

ASJC Scopus subject areas

  • Biochemistry

Cite this

The cysteine-peptidase bleomycin hydrolase is a member of the galactose regulon in yeast. / Zheng, Wenjin; Xu, H. Eric; Johnston, Stephen.

In: Journal of Biological Chemistry, Vol. 272, No. 48, 28.11.1997, p. 30350-30355.

Research output: Contribution to journalArticle

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