The cryo-EM method microcrystal electron diffraction (MicroED)

Brent Nannenga, Tamir Gonen

Research output: Contribution to journalReview article

4 Citations (Scopus)

Abstract

In 2013 we established a cryo-electron microscopy (cryo-EM) technique called microcrystal electron diffraction (MicroED). Since that time, data collection and analysis schemes have been fine-tuned, and structures for more than 40 different proteins, oligopeptides and organic molecules have been determined. Here we review the MicroED technique and place it in context with other structure-determination methods. We showcase example structures solved by MicroED and provide practical advice to prospective users.

Original languageEnglish (US)
Pages (from-to)369-379
Number of pages11
JournalNature Methods
Volume16
Issue number5
DOIs
StatePublished - May 1 2019

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Cryoelectron Microscopy
Microcrystals
Electron diffraction
Electron microscopy
Electrons
Oligopeptides
Molecules
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The cryo-EM method microcrystal electron diffraction (MicroED). / Nannenga, Brent; Gonen, Tamir.

In: Nature Methods, Vol. 16, No. 5, 01.05.2019, p. 369-379.

Research output: Contribution to journalReview article

Nannenga, Brent ; Gonen, Tamir. / The cryo-EM method microcrystal electron diffraction (MicroED). In: Nature Methods. 2019 ; Vol. 16, No. 5. pp. 369-379.
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