Abstract
In 2013 we established a cryo-electron microscopy (cryo-EM) technique called microcrystal electron diffraction (MicroED). Since that time, data collection and analysis schemes have been fine-tuned, and structures for more than 40 different proteins, oligopeptides and organic molecules have been determined. Here we review the MicroED technique and place it in context with other structure-determination methods. We showcase example structures solved by MicroED and provide practical advice to prospective users.
Original language | English (US) |
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Pages (from-to) | 369-379 |
Number of pages | 11 |
Journal | Nature Methods |
Volume | 16 |
Issue number | 5 |
DOIs | |
State | Published - May 1 2019 |
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Cell Biology