The Complete Protist Symbiont Communities of Coptotermes formosanus and Coptotermes gestroi: Morphological and Molecular Characterization of Five New Species

Daniel E. Jasso-Selles, Francesca De Martini, Joseph F. Velenovsky, Evan D. Mee, Samantha J. Montoya, Jonathon T. Hileman, Mikaela D. Garcia, Nan Yao Su, Thomas Chouvenc, Gillian H. Gile

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Coptotermes formosanus Shiraki and Coptotermes gestroi (Wasmann) (Blattoidea: Rhinotermitidae) are invasive subterranean termite pest species with a major global economic impact. However, the descriptions of the mutualistic protist communities harbored in their respective hindguts remain fragmentary. The C. formosanus hindgut has long been considered to harbor three protist species, Pseudotrichonympha grassii (Trichonymphida), Holomastigotoides hartmanni, and Cononympha (Spirotrichonympha) leidyi (Spirotrichonymphida), but molecular data have suggested that the diversity may be higher. Meanwhile, the C. gestroi community remains undescribed except for Pseudotrichonympha leei. To complete the characterization of these communities, hindguts of workers from both termite species were investigated using single-cell PCR, microscopy, cell counts, and 18S rRNA amplicon sequencing. The two hosts were found to harbor intriguingly parallel protist communities, each consisting of one Pseudotrichonympha species, two Holomastigotoides species, and two Cononympha species. All protist species were unique to their respective hosts, which last shared a common ancestor ~18 MYA. The relative abundances of protist species in each hindgut differed remarkably between cell count data and 18S rRNA profiles, calling for caution in interpreting species abundances from amplicon data. This study will enable future research in C. formosanus and C. gestroi hybrids, which provide a unique opportunity to study protist community inheritance, compatibility, and potential contribution to hybrid vigor.

Original languageEnglish (US)
Pages (from-to)626-641
Number of pages16
JournalJournal of Eukaryotic Microbiology
Volume67
Issue number6
DOIs
StatePublished - Nov 1 2020

Keywords

  • Cononympha
  • Holomastigotoides
  • Metamonada
  • Parabasalia
  • Pseudotrichonympha
  • Rhinotermitidae
  • Spirotrichonympha
  • hybrid
  • microbiome
  • termite

ASJC Scopus subject areas

  • Microbiology

Fingerprint Dive into the research topics of 'The Complete Protist Symbiont Communities of Coptotermes formosanus and Coptotermes gestroi: Morphological and Molecular Characterization of Five New Species'. Together they form a unique fingerprint.

Cite this