@article{b7ba8e5566e4469a98dbfe16c75ed450,
title = "The complete genome sequence of the staphylococcus bacteriophage metroid",
abstract = "Phages infecting bacteria of the genus Staphylococcus play an important role in their host's ecology and evolution. On one hand, horizontal gene transfer from phage can encourage the rapid adaptation of pathogenic Staphylococcus enabling them to escape host immunity or access novel environments. On the other hand, lytic phages are promising agents for the treatment of bacterial infections, especially those resistant to antibiotics. As part of an ongoing effort to gain novel insights into bacteriophage diversity, we characterized the complete genome of the Staphylococcus bacteriophage Metroid, a cluster C phage with a genome size of 151kb, encompassing 254 predicted protein-coding genes as well as 4 tRNAs. A comparative genomic analysis highlights strong similarities - including a conservation of the lysis cassette - with other Staphylococcus cluster C bacteriophages, several of which were previously characterized for therapeutic applications.",
keywords = "Assembly, Bacteriophage, De novo, Gene annotation, Myoviridae",
author = "Adele Crane and Joy Abaidoo and Gabriella Beltran and Danielle Fry and Colleen Furey and Noe Green and Ravneet Johal and {la Rosa}, Bruno and Jimenez, {Catalina Lopez} and Linh Luong and Garett Maag and Jade Porche and Lauren Reyes and Aspen Robinson and Samantha Sabbara and Herrera, {Lucia Soto} and Negrete, {Angelica Urquidez} and Pauline Wilson and Kerry Geiler-Samerotte and Pfeifer, {Susanne P.}",
note = "Funding Information: This study was supported by the Howard Hughes Medical Institute SEA-PHAGES program and Arizona State University's School of Life Sciences. DNA concentration was determined in the Arizona State University DNA Shared Resource Facility. Library preparation and sequencing was performed at the University of Pittsburgh. Computations were partially performed at Arizona State University's High Performance Computing facility. We are grateful to David Lowry for transmission electron microscopy imaging, Suhail Ghafoor for IT support, Billy Biederman, Graham Hatfull, Deborah Jacobs-Sera, Welkin Pope, Daniel Russell, and Vic Sivanathan for library preparation, sequencing, and assembly as well as providing faculty training for the SEA-PHAGES program and guidance with our genome annotation. Funding Information: This study was supported by the Howard Hughes Medical Institute SEA-PHAGES program and Arizona State University{\textquoteright}s School of Life Sciences. DNA concentration was determined in the Arizona State University DNA Shared Resource Facility. Library preparation and sequencing was performed at the University of Pittsburgh. Computations were partially performed at Arizona State University{\textquoteright}s High Performance Computing facility. We are grateful to David Lowry for transmission electron microscopy imaging, Suhail Ghafoor for IT support, Billy Biederman, Graham Hatfull, Deborah Jacobs-Sera, Welkin Pope, Daniel Russell, and Vic Sivanathan for library preparation, sequencing, and assembly as well as providing faculty training for the SEA-PHAGES program and guidance with our genome annotation. Publisher Copyright: Copyright {\textcopyright} 2020 Crane et al.",
year = "2020",
month = sep,
day = "1",
doi = "10.1534/g3.120.401365",
language = "English (US)",
volume = "10",
pages = "2975--2979",
journal = "G3 (Bethesda, Md.)",
issn = "2160-1836",
publisher = "Genetics Society of America",
number = "9",
}