The autologous mixed lymphocyte reaction in primary biliary cirrhosis

Analysis of activation and blastogenesis of autoreactive T lymphocytes

Keith Lindor, R. H. Wiesner, E. R. Dickson, H. A. Homburger

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Blastogenesis of autoreactive T lymphocytes in the autologous mixed lymphocyte reaction has been shown to be decreased in patients with primary biliary cirrhosis, but the mechanisms underlying this abnormality are not known. To investigate further the abnormal autologous mixed lymphocyte reaction in primary biliary cirrhosis, we measured the activation of autoreactive helper/inducer and suppressor/cytotoxic T lymphocytes concurrently with blastogenesis in 35 patients with primary biliary cirrhosis and 18 healthy controls. Blastogenesis was diminished in autologous mixed lymphocyte reaction cultures from primary biliary cirrhosis patients compared to the control subjects (25,273 ± 20,259 dpm vs. 36,004 ± 14,951 dpm, p < 0.02), but cultures from patients with primary biliary cirrhosis contained significantly increased percentages of activated helper/inducer and suppressor/cytotoxic T lymphocytes: 20.6 ± 7.9 vs. 15.5 ± 5.3%, p < 0.008, and 9.8 ± 7.8 vs. 5.4 ± 3.0%, p < 0.02, respectively. These findings were not related to the histologic stage of liver disease or to the serum bilirubin concentration and were not associated with abnormalities of lymphocyte subsets in fresh peripheral blood specimens. We conclude that the percentage of autoreactive T lymphocytes in autologous mixed lymphocyte reaction cultures from peripheral blood is increased in patients with primary biliary cirrhosis and diminished blastogenesis in autologous mixed lymphocyte reaction cultures is not due to the loss of autoreactive T lymphoctes from peripheral blood. Diminished blastogenesis reflects a diminished proliferative response of activated, autoreactive T lymphocytes in primary biliary cirrhosis. Possible mechanisms that may account for the paradoxical findings of decreased blastogenesis and increased activation of autoreactive T lymphocytes in primary biliary cirrhosis are discussed.

Original languageEnglish (US)
Pages (from-to)1555-1559
Number of pages5
JournalHepatology
Volume8
Issue number6
StatePublished - 1988
Externally publishedYes

Fingerprint

Activation Analysis
Mixed Lymphocyte Culture Test
Biliary Liver Cirrhosis
Lymphocyte Activation
T-Lymphocytes
Cytotoxic T-Lymphocytes
Lymphocyte Subsets
Bilirubin
Liver Diseases

ASJC Scopus subject areas

  • Hepatology

Cite this

The autologous mixed lymphocyte reaction in primary biliary cirrhosis : Analysis of activation and blastogenesis of autoreactive T lymphocytes. / Lindor, Keith; Wiesner, R. H.; Dickson, E. R.; Homburger, H. A.

In: Hepatology, Vol. 8, No. 6, 1988, p. 1555-1559.

Research output: Contribution to journalArticle

@article{dc6db4ab646c4338b8c824475c3f480f,
title = "The autologous mixed lymphocyte reaction in primary biliary cirrhosis: Analysis of activation and blastogenesis of autoreactive T lymphocytes",
abstract = "Blastogenesis of autoreactive T lymphocytes in the autologous mixed lymphocyte reaction has been shown to be decreased in patients with primary biliary cirrhosis, but the mechanisms underlying this abnormality are not known. To investigate further the abnormal autologous mixed lymphocyte reaction in primary biliary cirrhosis, we measured the activation of autoreactive helper/inducer and suppressor/cytotoxic T lymphocytes concurrently with blastogenesis in 35 patients with primary biliary cirrhosis and 18 healthy controls. Blastogenesis was diminished in autologous mixed lymphocyte reaction cultures from primary biliary cirrhosis patients compared to the control subjects (25,273 ± 20,259 dpm vs. 36,004 ± 14,951 dpm, p < 0.02), but cultures from patients with primary biliary cirrhosis contained significantly increased percentages of activated helper/inducer and suppressor/cytotoxic T lymphocytes: 20.6 ± 7.9 vs. 15.5 ± 5.3{\%}, p < 0.008, and 9.8 ± 7.8 vs. 5.4 ± 3.0{\%}, p < 0.02, respectively. These findings were not related to the histologic stage of liver disease or to the serum bilirubin concentration and were not associated with abnormalities of lymphocyte subsets in fresh peripheral blood specimens. We conclude that the percentage of autoreactive T lymphocytes in autologous mixed lymphocyte reaction cultures from peripheral blood is increased in patients with primary biliary cirrhosis and diminished blastogenesis in autologous mixed lymphocyte reaction cultures is not due to the loss of autoreactive T lymphoctes from peripheral blood. Diminished blastogenesis reflects a diminished proliferative response of activated, autoreactive T lymphocytes in primary biliary cirrhosis. Possible mechanisms that may account for the paradoxical findings of decreased blastogenesis and increased activation of autoreactive T lymphocytes in primary biliary cirrhosis are discussed.",
author = "Keith Lindor and Wiesner, {R. H.} and Dickson, {E. R.} and Homburger, {H. A.}",
year = "1988",
language = "English (US)",
volume = "8",
pages = "1555--1559",
journal = "Hepatology",
issn = "0270-9139",
publisher = "John Wiley and Sons Ltd",
number = "6",

}

TY - JOUR

T1 - The autologous mixed lymphocyte reaction in primary biliary cirrhosis

T2 - Analysis of activation and blastogenesis of autoreactive T lymphocytes

AU - Lindor, Keith

AU - Wiesner, R. H.

AU - Dickson, E. R.

AU - Homburger, H. A.

PY - 1988

Y1 - 1988

N2 - Blastogenesis of autoreactive T lymphocytes in the autologous mixed lymphocyte reaction has been shown to be decreased in patients with primary biliary cirrhosis, but the mechanisms underlying this abnormality are not known. To investigate further the abnormal autologous mixed lymphocyte reaction in primary biliary cirrhosis, we measured the activation of autoreactive helper/inducer and suppressor/cytotoxic T lymphocytes concurrently with blastogenesis in 35 patients with primary biliary cirrhosis and 18 healthy controls. Blastogenesis was diminished in autologous mixed lymphocyte reaction cultures from primary biliary cirrhosis patients compared to the control subjects (25,273 ± 20,259 dpm vs. 36,004 ± 14,951 dpm, p < 0.02), but cultures from patients with primary biliary cirrhosis contained significantly increased percentages of activated helper/inducer and suppressor/cytotoxic T lymphocytes: 20.6 ± 7.9 vs. 15.5 ± 5.3%, p < 0.008, and 9.8 ± 7.8 vs. 5.4 ± 3.0%, p < 0.02, respectively. These findings were not related to the histologic stage of liver disease or to the serum bilirubin concentration and were not associated with abnormalities of lymphocyte subsets in fresh peripheral blood specimens. We conclude that the percentage of autoreactive T lymphocytes in autologous mixed lymphocyte reaction cultures from peripheral blood is increased in patients with primary biliary cirrhosis and diminished blastogenesis in autologous mixed lymphocyte reaction cultures is not due to the loss of autoreactive T lymphoctes from peripheral blood. Diminished blastogenesis reflects a diminished proliferative response of activated, autoreactive T lymphocytes in primary biliary cirrhosis. Possible mechanisms that may account for the paradoxical findings of decreased blastogenesis and increased activation of autoreactive T lymphocytes in primary biliary cirrhosis are discussed.

AB - Blastogenesis of autoreactive T lymphocytes in the autologous mixed lymphocyte reaction has been shown to be decreased in patients with primary biliary cirrhosis, but the mechanisms underlying this abnormality are not known. To investigate further the abnormal autologous mixed lymphocyte reaction in primary biliary cirrhosis, we measured the activation of autoreactive helper/inducer and suppressor/cytotoxic T lymphocytes concurrently with blastogenesis in 35 patients with primary biliary cirrhosis and 18 healthy controls. Blastogenesis was diminished in autologous mixed lymphocyte reaction cultures from primary biliary cirrhosis patients compared to the control subjects (25,273 ± 20,259 dpm vs. 36,004 ± 14,951 dpm, p < 0.02), but cultures from patients with primary biliary cirrhosis contained significantly increased percentages of activated helper/inducer and suppressor/cytotoxic T lymphocytes: 20.6 ± 7.9 vs. 15.5 ± 5.3%, p < 0.008, and 9.8 ± 7.8 vs. 5.4 ± 3.0%, p < 0.02, respectively. These findings were not related to the histologic stage of liver disease or to the serum bilirubin concentration and were not associated with abnormalities of lymphocyte subsets in fresh peripheral blood specimens. We conclude that the percentage of autoreactive T lymphocytes in autologous mixed lymphocyte reaction cultures from peripheral blood is increased in patients with primary biliary cirrhosis and diminished blastogenesis in autologous mixed lymphocyte reaction cultures is not due to the loss of autoreactive T lymphoctes from peripheral blood. Diminished blastogenesis reflects a diminished proliferative response of activated, autoreactive T lymphocytes in primary biliary cirrhosis. Possible mechanisms that may account for the paradoxical findings of decreased blastogenesis and increased activation of autoreactive T lymphocytes in primary biliary cirrhosis are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0024212266&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024212266&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 1555

EP - 1559

JO - Hepatology

JF - Hepatology

SN - 0270-9139

IS - 6

ER -