The attenuated NYCBH vaccinia virus deleted for the immune evasion gene, E3L, completely protects mice against heterologous challenge with ectromelia virus

Karen L. Denzler, Jill Schriewer, Scott Parker, Chas Werner, Hollyce Hartzler, Ed Hembrador, Trung Huynh, Susan Holechek, R. M. Buller, Bertram Jacobs

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The New York City Board of Health (NYCBH) vaccinia virus (VACV) vaccine strain was deleted for the immune evasion gene, E3L, and tested for its pathogenicity and ability to protect mice from heterologous challenge with ectromelia virus (ECTV). NYCBHΔE3L was found to be highly attenuated for pathogenicity in a newborn mouse model and showed a similar attenuated phenotype as the NYVAC strain of vaccinia virus. Scarification with one or two doses of the attenuated NYCBHΔE3L was able to protect mice equally as well as NYCBH from death, weight loss, and viral spread to visceral organs. A single dose of NYCBHΔE3L resulted in low poxvirus-specific antibodies, and a second dose increased levels of poxvirus-specific antibodies to a level similar to that seen in animals vaccinated with a single dose of NYCBH. However, similar neutralizing antibody titers were observed following one or two doses of NYCBHΔE3L or NYCBH. Thus, NYCBHΔE3L shows potential as a candidate for a safer human smallpox vaccine since it protects mice from challenge with a heterologous poxvirus.

Original languageEnglish (US)
Pages (from-to)9691-9696
Number of pages6
JournalVaccine
Volume29
Issue number52
DOIs
StatePublished - Dec 6 2011

Fingerprint

Ectromelia virus
immune evasion
Immune Evasion
Vaccinia virus
Poxviridae
mice
Health
dosage
Genes
Virulence
genes
Smallpox Vaccine
pathogenicity
Antibodies
vaccines
Neutralizing Antibodies
antibodies
Weight Loss
neutralizing antibodies
Vaccines

Keywords

  • E3L
  • Ectromelia virus
  • Smallpox
  • Vaccinia virus

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

The attenuated NYCBH vaccinia virus deleted for the immune evasion gene, E3L, completely protects mice against heterologous challenge with ectromelia virus. / Denzler, Karen L.; Schriewer, Jill; Parker, Scott; Werner, Chas; Hartzler, Hollyce; Hembrador, Ed; Huynh, Trung; Holechek, Susan; Buller, R. M.; Jacobs, Bertram.

In: Vaccine, Vol. 29, No. 52, 06.12.2011, p. 9691-9696.

Research output: Contribution to journalArticle

Denzler, KL, Schriewer, J, Parker, S, Werner, C, Hartzler, H, Hembrador, E, Huynh, T, Holechek, S, Buller, RM & Jacobs, B 2011, 'The attenuated NYCBH vaccinia virus deleted for the immune evasion gene, E3L, completely protects mice against heterologous challenge with ectromelia virus', Vaccine, vol. 29, no. 52, pp. 9691-9696. https://doi.org/10.1016/j.vaccine.2011.09.108
Denzler, Karen L. ; Schriewer, Jill ; Parker, Scott ; Werner, Chas ; Hartzler, Hollyce ; Hembrador, Ed ; Huynh, Trung ; Holechek, Susan ; Buller, R. M. ; Jacobs, Bertram. / The attenuated NYCBH vaccinia virus deleted for the immune evasion gene, E3L, completely protects mice against heterologous challenge with ectromelia virus. In: Vaccine. 2011 ; Vol. 29, No. 52. pp. 9691-9696.
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