The actin motor MYO-5 effect in the intracellular organization of Neurospora crassa

Arianne Ramírez-del Villar, Robert Roberson, Olga A. Callejas-Negrete, Rosa R. Mouriño-Pérez

    Research output: Contribution to journalArticle

    Abstract

    In filamentous fungi, polarized growth is the result of vesicle secretion at the hyphal apex. Motor proteins mediate vesicle transport to target destinations on the plasma membrane via actin and microtubule cytoskeletons. Myosins are motor proteins associated with actin filaments. Specifically, class V myosins are responsible for cargo transport in eukaryotes. We studied the dynamics and localization of myosin V in wild type hyphae of Neurospora crassa and in hyphae that lacked MYO-5. In wild type hyphae, MYO-5-GFP was localized concentrated in the hyphal apex and colocalized with Spitzenkörper. Photobleaching studies showed that MYO-5-GFP was transported to the apex from subapical hyphal regions. The deletion of the class V myosin resulted in a reduced rate of hyphal growth, apical hyperbranching, and intermittent loss of hyphal polarity. MYO-5 did not participate in breaking the symmetrical growth during germination but contributed in the apical organization upon establishment of polarized growth. In the Δmyo-5 mutant, actin was organized into thick cables in the apical and subapical hyphal regions, and the number of endocytic patches was reduced. The microvesicles-chitosomes observed with CHS-1-GFP were distributed as a cloud occupying the apical dome and not in the Spitzenkörper as the WT strain. The mitochondrial movement was not associated with MYO-5, but tubular vacuole position is MYO-5-dependent. These results suggest that MYO-5 plays a role in maintaining apical organization and the integrity of the Spitzenkörper and is required for normal hyphal growth, polarity, septation, conidiation, and proper conidial germination.

    Original languageEnglish (US)
    Pages (from-to)13-27
    Number of pages15
    JournalFungal Genetics and Biology
    Volume125
    DOIs
    StatePublished - Apr 1 2019

    Fingerprint

    Neurospora crassa
    Myosin Type V
    Actins
    Hyphae
    Growth
    Germination
    Actin Cytoskeleton
    Photobleaching
    Transport Vesicles
    Myosins
    Vacuoles
    Eukaryota
    Microtubules
    Proteins
    Fungi
    Cell Membrane

    Keywords

    • Actin
    • Class V myosin
    • MYO-5
    • Neursopora crassa
    • Vacuoles

    ASJC Scopus subject areas

    • Microbiology
    • Genetics

    Cite this

    The actin motor MYO-5 effect in the intracellular organization of Neurospora crassa. / Ramírez-del Villar, Arianne; Roberson, Robert; Callejas-Negrete, Olga A.; Mouriño-Pérez, Rosa R.

    In: Fungal Genetics and Biology, Vol. 125, 01.04.2019, p. 13-27.

    Research output: Contribution to journalArticle

    Ramírez-del Villar, Arianne ; Roberson, Robert ; Callejas-Negrete, Olga A. ; Mouriño-Pérez, Rosa R. / The actin motor MYO-5 effect in the intracellular organization of Neurospora crassa. In: Fungal Genetics and Biology. 2019 ; Vol. 125. pp. 13-27.
    @article{26929cfc02ef45bd95fb830125a76415,
    title = "The actin motor MYO-5 effect in the intracellular organization of Neurospora crassa",
    abstract = "In filamentous fungi, polarized growth is the result of vesicle secretion at the hyphal apex. Motor proteins mediate vesicle transport to target destinations on the plasma membrane via actin and microtubule cytoskeletons. Myosins are motor proteins associated with actin filaments. Specifically, class V myosins are responsible for cargo transport in eukaryotes. We studied the dynamics and localization of myosin V in wild type hyphae of Neurospora crassa and in hyphae that lacked MYO-5. In wild type hyphae, MYO-5-GFP was localized concentrated in the hyphal apex and colocalized with Spitzenk{\"o}rper. Photobleaching studies showed that MYO-5-GFP was transported to the apex from subapical hyphal regions. The deletion of the class V myosin resulted in a reduced rate of hyphal growth, apical hyperbranching, and intermittent loss of hyphal polarity. MYO-5 did not participate in breaking the symmetrical growth during germination but contributed in the apical organization upon establishment of polarized growth. In the Δmyo-5 mutant, actin was organized into thick cables in the apical and subapical hyphal regions, and the number of endocytic patches was reduced. The microvesicles-chitosomes observed with CHS-1-GFP were distributed as a cloud occupying the apical dome and not in the Spitzenk{\"o}rper as the WT strain. The mitochondrial movement was not associated with MYO-5, but tubular vacuole position is MYO-5-dependent. These results suggest that MYO-5 plays a role in maintaining apical organization and the integrity of the Spitzenk{\"o}rper and is required for normal hyphal growth, polarity, septation, conidiation, and proper conidial germination.",
    keywords = "Actin, Class V myosin, MYO-5, Neursopora crassa, Vacuoles",
    author = "{Ram{\'i}rez-del Villar}, Arianne and Robert Roberson and Callejas-Negrete, {Olga A.} and Mouri{\~n}o-P{\'e}rez, {Rosa R.}",
    year = "2019",
    month = "4",
    day = "1",
    doi = "10.1016/j.fgb.2018.11.008",
    language = "English (US)",
    volume = "125",
    pages = "13--27",
    journal = "Fungal Genetics and Biology",
    issn = "1087-1845",
    publisher = "Academic Press Inc.",

    }

    TY - JOUR

    T1 - The actin motor MYO-5 effect in the intracellular organization of Neurospora crassa

    AU - Ramírez-del Villar, Arianne

    AU - Roberson, Robert

    AU - Callejas-Negrete, Olga A.

    AU - Mouriño-Pérez, Rosa R.

    PY - 2019/4/1

    Y1 - 2019/4/1

    N2 - In filamentous fungi, polarized growth is the result of vesicle secretion at the hyphal apex. Motor proteins mediate vesicle transport to target destinations on the plasma membrane via actin and microtubule cytoskeletons. Myosins are motor proteins associated with actin filaments. Specifically, class V myosins are responsible for cargo transport in eukaryotes. We studied the dynamics and localization of myosin V in wild type hyphae of Neurospora crassa and in hyphae that lacked MYO-5. In wild type hyphae, MYO-5-GFP was localized concentrated in the hyphal apex and colocalized with Spitzenkörper. Photobleaching studies showed that MYO-5-GFP was transported to the apex from subapical hyphal regions. The deletion of the class V myosin resulted in a reduced rate of hyphal growth, apical hyperbranching, and intermittent loss of hyphal polarity. MYO-5 did not participate in breaking the symmetrical growth during germination but contributed in the apical organization upon establishment of polarized growth. In the Δmyo-5 mutant, actin was organized into thick cables in the apical and subapical hyphal regions, and the number of endocytic patches was reduced. The microvesicles-chitosomes observed with CHS-1-GFP were distributed as a cloud occupying the apical dome and not in the Spitzenkörper as the WT strain. The mitochondrial movement was not associated with MYO-5, but tubular vacuole position is MYO-5-dependent. These results suggest that MYO-5 plays a role in maintaining apical organization and the integrity of the Spitzenkörper and is required for normal hyphal growth, polarity, septation, conidiation, and proper conidial germination.

    AB - In filamentous fungi, polarized growth is the result of vesicle secretion at the hyphal apex. Motor proteins mediate vesicle transport to target destinations on the plasma membrane via actin and microtubule cytoskeletons. Myosins are motor proteins associated with actin filaments. Specifically, class V myosins are responsible for cargo transport in eukaryotes. We studied the dynamics and localization of myosin V in wild type hyphae of Neurospora crassa and in hyphae that lacked MYO-5. In wild type hyphae, MYO-5-GFP was localized concentrated in the hyphal apex and colocalized with Spitzenkörper. Photobleaching studies showed that MYO-5-GFP was transported to the apex from subapical hyphal regions. The deletion of the class V myosin resulted in a reduced rate of hyphal growth, apical hyperbranching, and intermittent loss of hyphal polarity. MYO-5 did not participate in breaking the symmetrical growth during germination but contributed in the apical organization upon establishment of polarized growth. In the Δmyo-5 mutant, actin was organized into thick cables in the apical and subapical hyphal regions, and the number of endocytic patches was reduced. The microvesicles-chitosomes observed with CHS-1-GFP were distributed as a cloud occupying the apical dome and not in the Spitzenkörper as the WT strain. The mitochondrial movement was not associated with MYO-5, but tubular vacuole position is MYO-5-dependent. These results suggest that MYO-5 plays a role in maintaining apical organization and the integrity of the Spitzenkörper and is required for normal hyphal growth, polarity, septation, conidiation, and proper conidial germination.

    KW - Actin

    KW - Class V myosin

    KW - MYO-5

    KW - Neursopora crassa

    KW - Vacuoles

    UR - http://www.scopus.com/inward/record.url?scp=85060248260&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=85060248260&partnerID=8YFLogxK

    U2 - 10.1016/j.fgb.2018.11.008

    DO - 10.1016/j.fgb.2018.11.008

    M3 - Article

    VL - 125

    SP - 13

    EP - 27

    JO - Fungal Genetics and Biology

    JF - Fungal Genetics and Biology

    SN - 1087-1845

    ER -