The 19S regulatory particle of the proteasome is required for efficient transcription elongation by RNA polymerase II

Anwarul Ferdous, Fernando Gonzalez, Liping Sun, Thomas Kodadek, Stephen Albert Johnston

Research output: Contribution to journalArticlepeer-review

229 Scopus citations

Abstract

It is generally thought that the primary or even sole activity of the 19S regulatory particle of the 26S proteasome is to facilitate the degradation of polyubiquitinated proteins by the 20S-core subunit. However, we present evidence that the 19S complex is required for efficient elongation of RNA polymerase II (RNAP II) in vitro and in vivo. First, yeast strains carrying alleles of SUG1 and SUG2, encoding 19S components, exhibit phenotypes indicative of elongation defects. Second, in vitro transcription is inhibited by antibodies raised against Sug1, or by heat-inactivating temperature-sensitive Sug1 mutants with restoration of elongation by addition of immunopurified 19S complex. Finally, Cdc68, a known elongation factor, coimmunoprecipitates with the 19S complex, indicating a physical interaction. Inhibition of the 20S proteolytic core of the proteasome has no effect on elongation. This work defines a nonproteolytic role for the 19S complex in RNAP II transcription.

Original languageEnglish (US)
Pages (from-to)981-991
Number of pages11
JournalMolecular Cell
Volume7
Issue number5
DOIs
StatePublished - May 25 2001
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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