Tentacle Probes™: Differentiation of difficult single-nucleotide polymorphisms and deletions by presence or absence of a signal in real-time PCR

Brent C. Satterfield, David A. Kulesh, David A. Norwood, Leonard P. Wasieloski, Michael Caplan, Jay A A West

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: False-positive results are a common problem in real-time PCR identification of DNA sequences that differ from near neighbors by a single-nucleotide polymorphism (SNP) or deletion. Because of a lack of sufficient probe specificity, post-PCR analysis, such as a melting curve, is often required for mutation differentiation. Methods: Tentacle Probes™, cooperative reagents with both a capture and a detection probe based on specific cell-targeting principles, were developed as a replacement for 2 chromosomal TaqMan-minor groove binder (MGB) assays previously developed for Yersinia pestis and Bacillus anthracis detection. We compared TaqMan-MGB probes to Tentacle Probes for SNP and deletion detection based on the presence or absence of a growth curve. Results: With the TaqMan-MGB Y. pestis yp48 assays, false-positive results for Yersinia pseudotuberculosis occurred at every concentration tested, and with the TaqMan-MGB B. anthracis gyrA assays, false-positive results occurred in 21 of 29 boil preps of environmental samples of near neighbors. With Tentacle Probes no false-positive results occurred. Conclusions: The high specificity exhibited by Tentacle Probes may eliminate melting curve analysis for SNP and deletion mutation detection, allowing the diagnostic use of previously difficult targets.

Original languageEnglish (US)
Pages (from-to)2042-2050
Number of pages9
JournalClinical Chemistry
Volume53
Issue number12
DOIs
StatePublished - Dec 2007

Fingerprint

Polymorphism
Single Nucleotide Polymorphism
Yersinia pestis
Bacillus anthracis
Real-Time Polymerase Chain Reaction
Nucleotides
Freezing
Binders
Yersinia pseudotuberculosis
Furunculosis
Sequence Deletion
Assays
Melting
Polymerase Chain Reaction
Mutation
Growth
DNA sequences
Bacilli

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Tentacle Probes™ : Differentiation of difficult single-nucleotide polymorphisms and deletions by presence or absence of a signal in real-time PCR. / Satterfield, Brent C.; Kulesh, David A.; Norwood, David A.; Wasieloski, Leonard P.; Caplan, Michael; West, Jay A A.

In: Clinical Chemistry, Vol. 53, No. 12, 12.2007, p. 2042-2050.

Research output: Contribution to journalArticle

Satterfield, Brent C. ; Kulesh, David A. ; Norwood, David A. ; Wasieloski, Leonard P. ; Caplan, Michael ; West, Jay A A. / Tentacle Probes™ : Differentiation of difficult single-nucleotide polymorphisms and deletions by presence or absence of a signal in real-time PCR. In: Clinical Chemistry. 2007 ; Vol. 53, No. 12. pp. 2042-2050.
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