Temporal differences in Erk1/2 activity distinguish among combinations of extracellular matrix components

Christine M. Pauken, Michael Caplan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Rational design of biomaterials requires understanding how cells interrogate their microenvironment. In this study, human umbilical vein endothelial cells are cultured on combinations of extracellular matrix (ECM) components (collagen I, collagen IV, vitronectin, fibronectin, laminin, heparan sulfate proteoglycan, chondroitin sulfate proteoglycan), and the phosphorylation of four intracellular signaling kinases (Erk1/2, JNK, Akt1, and NFκB) is quantified. These combinations of ECM components elicit different temporal patterns of Erk1/2 phosphorylation. Collagen I-containing substrates cause Erk1/2 phosphorylation to reach maximal levels at 30 min and remain near maximal levels until 90 min. Collagen IV/laminin substrates elicit maximal phosphorylation at 30-45 min, and then phosphorylation decreases substantially at 60-90 min. All other combinations studied (collagen IV and vitronectin-based combinations) cause an increase in phosphorylation at 30-45 min, but not to maximal levels; maximal phosphorylation is reached by 60-90 min. These temporal patterns of phosphorylation may explain how a limited number of intracellular signaling pathways can distinguish among thousands of possible combinations of microenvironmental cues by adding to the information contained in each cell signaling pathway.

Original languageEnglish (US)
Pages (from-to)3973-3980
Number of pages8
JournalActa Biomaterialia
Volume7
Issue number11
DOIs
StatePublished - Nov 2011

Fingerprint

Phosphorylation
Extracellular Matrix
Collagen
Vitronectin
Laminin
Cell signaling
Chondroitin Sulfate Proteoglycans
Heparan Sulfate Proteoglycans
Endothelial cells
Human Umbilical Vein Endothelial Cells
Biocompatible Materials
Substrates
Fibronectins
Biomaterials
Cues
Phosphotransferases

Keywords

  • Basement membrane
  • Biomaterial
  • Endothelial cells
  • Intracellular signaling
  • Systems biology

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Temporal differences in Erk1/2 activity distinguish among combinations of extracellular matrix components. / Pauken, Christine M.; Caplan, Michael.

In: Acta Biomaterialia, Vol. 7, No. 11, 11.2011, p. 3973-3980.

Research output: Contribution to journalArticle

Pauken, Christine M. ; Caplan, Michael. / Temporal differences in Erk1/2 activity distinguish among combinations of extracellular matrix components. In: Acta Biomaterialia. 2011 ; Vol. 7, No. 11. pp. 3973-3980.
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