Template-directed synthesis of self-assembled inorganic nanostructures

Linda A. Stearns; Rahul Chhabra; William Petuskey; Hao Yan; John C. Chaput

Template-directed synthesis of self-assembled inorganic nanostructures

Linda A. Stearns, Rahul Chhabra, William Petuskey, Hao Yan, John C. Chaput

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The A3 peptide, discovered by in vitro evolution, has been shown to bind silver and gold nanoparticles. We chemically conjugated the A3 peptide to a 20-mer oligonucleotide, thereby generating an A3 peptide-oligonucleotide conjugate (A3 POC). The A3 POC bears the complementary sequence to 10 single-stranded DNA "capture" probes extending outward from the surface of an origami DNA fixed-length nanotube. After assembling the A3 POC-nanotube complex in Hepes buffer, AuCl4-was added to induce Au nanoparticle (NP) nucleation and growth. Transmission electron microscopy revealed that a majority of the fixed-length tubes were decorated with 5 to 6 Au NPs (~10 nm in diameter) each, while a negative control (no A3 POC) showed NPs that were not localized to the DNA tubes. At present, we are working to optimize Au NP nucleation to achieve the maximum of 10 NPs per tube, while minimizing salt-induced aggregation effects.

Original language	English (US)
Title of host publication	Extended Abstracts for 6th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2009
Pages	89-92
Number of pages	4
State	Published - 2009
Event	6th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2009 - Snowbird, UT, United States Duration: Apr 20 2009 → Apr 24 2009

Other

Other	6th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2009
Country/Territory	United States
City	Snowbird, UT
Period	4/20/09 → 4/24/09

ASJC Scopus subject areas

Hardware and Architecture
Electrical and Electronic Engineering

Cite this

Stearns, LA, Chhabra, R, Petuskey, W, Yan, H & Chaput, JC 2009, Template-directed synthesis of self-assembled inorganic nanostructures. in Extended Abstracts for 6th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2009. pp. 89-92, 6th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2009, Snowbird, UT, United States, 4/20/09.

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author = "Stearns, {Linda A.} and Rahul Chhabra and William Petuskey and Hao Yan and Chaput, {John C.}",

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AU - Stearns, Linda A.

AU - Chhabra, Rahul

AU - Petuskey, William

AU - Yan, Hao

AU - Chaput, John C.

PY - 2009

Y1 - 2009

N2 - The A3 peptide, discovered by in vitro evolution, has been shown to bind silver and gold nanoparticles. We chemically conjugated the A3 peptide to a 20-mer oligonucleotide, thereby generating an A3 peptide-oligonucleotide conjugate (A3 POC). The A3 POC bears the complementary sequence to 10 single-stranded DNA "capture" probes extending outward from the surface of an origami DNA fixed-length nanotube. After assembling the A3 POC-nanotube complex in Hepes buffer, AuCl4-was added to induce Au nanoparticle (NP) nucleation and growth. Transmission electron microscopy revealed that a majority of the fixed-length tubes were decorated with 5 to 6 Au NPs (~10 nm in diameter) each, while a negative control (no A3 POC) showed NPs that were not localized to the DNA tubes. At present, we are working to optimize Au NP nucleation to achieve the maximum of 10 NPs per tube, while minimizing salt-induced aggregation effects.

AB - The A3 peptide, discovered by in vitro evolution, has been shown to bind silver and gold nanoparticles. We chemically conjugated the A3 peptide to a 20-mer oligonucleotide, thereby generating an A3 peptide-oligonucleotide conjugate (A3 POC). The A3 POC bears the complementary sequence to 10 single-stranded DNA "capture" probes extending outward from the surface of an origami DNA fixed-length nanotube. After assembling the A3 POC-nanotube complex in Hepes buffer, AuCl4-was added to induce Au nanoparticle (NP) nucleation and growth. Transmission electron microscopy revealed that a majority of the fixed-length tubes were decorated with 5 to 6 Au NPs (~10 nm in diameter) each, while a negative control (no A3 POC) showed NPs that were not localized to the DNA tubes. At present, we are working to optimize Au NP nucleation to achieve the maximum of 10 NPs per tube, while minimizing salt-induced aggregation effects.

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T2 - 6th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2009

Y2 - 20 April 2009 through 24 April 2009

ER -

Template-directed synthesis of self-assembled inorganic nanostructures

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