Temperature effects on intra- and extracellular acid-base status in the American locust, Schistocerca nitens

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Abstract

This study examined the effect of temperature on hemolymph and intracellular acid-base status in the locust, Schistocerca nitens. Hemolymph pH decreased with temperature by 0.017 pH units·°C-1 above 25°C, but was stable at lower temperatures. Average intracellular pH (pHi, calculated from CO2 distribution) decreased by approximately 0.018 pH units·°C-1, in accordance with predictions for preservation of relative alkalinity and protein charge state. DMO was found to be unsuitable for use as an in vivo pHi marker in locusts due to rapid metabolism and excretion of this compound. Hemolymph pH regulation when temperature changed was accomplished by a combination of variation of PCO2 and [HCO3 -], with changes in [HCO3 -] predominating. Digestive tracts contained a large portion of total body water (over 30%), and total body CO2 (over 40%). Variation in [HCO3 -] may dominate pH regulation in locusts because of the relatively large size of the digestive tract and its powerful acid-base transporting capacities.

Original languageEnglish (US)
Pages (from-to)763-770
Number of pages8
JournalJournal of Comparative Physiology B
Volume158
Issue number6
DOIs
StatePublished - Nov 1989
Externally publishedYes

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Schistocerca nitens
Grasshoppers
locust
locusts
temperature effect
Thermal effects
Temperature
Acids
Hemolymph
acids
acid
hemolymph
temperature
digestive tract
excretion
alkalinity
Gastrointestinal Tract
Alkalinity
metabolism
Metabolism

ASJC Scopus subject areas

  • Physiology (medical)
  • Environmental Science(all)
  • Physiology
  • Animal Science and Zoology

Cite this

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title = "Temperature effects on intra- and extracellular acid-base status in the American locust, Schistocerca nitens",
abstract = "This study examined the effect of temperature on hemolymph and intracellular acid-base status in the locust, Schistocerca nitens. Hemolymph pH decreased with temperature by 0.017 pH units·°C-1 above 25°C, but was stable at lower temperatures. Average intracellular pH (pHi, calculated from CO2 distribution) decreased by approximately 0.018 pH units·°C-1, in accordance with predictions for preservation of relative alkalinity and protein charge state. DMO was found to be unsuitable for use as an in vivo pHi marker in locusts due to rapid metabolism and excretion of this compound. Hemolymph pH regulation when temperature changed was accomplished by a combination of variation of PCO2 and [HCO3 -], with changes in [HCO3 -] predominating. Digestive tracts contained a large portion of total body water (over 30{\%}), and total body CO2 (over 40{\%}). Variation in [HCO3 -] may dominate pH regulation in locusts because of the relatively large size of the digestive tract and its powerful acid-base transporting capacities.",
author = "Jon Harrison",
year = "1989",
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T1 - Temperature effects on intra- and extracellular acid-base status in the American locust, Schistocerca nitens

AU - Harrison, Jon

PY - 1989/11

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N2 - This study examined the effect of temperature on hemolymph and intracellular acid-base status in the locust, Schistocerca nitens. Hemolymph pH decreased with temperature by 0.017 pH units·°C-1 above 25°C, but was stable at lower temperatures. Average intracellular pH (pHi, calculated from CO2 distribution) decreased by approximately 0.018 pH units·°C-1, in accordance with predictions for preservation of relative alkalinity and protein charge state. DMO was found to be unsuitable for use as an in vivo pHi marker in locusts due to rapid metabolism and excretion of this compound. Hemolymph pH regulation when temperature changed was accomplished by a combination of variation of PCO2 and [HCO3 -], with changes in [HCO3 -] predominating. Digestive tracts contained a large portion of total body water (over 30%), and total body CO2 (over 40%). Variation in [HCO3 -] may dominate pH regulation in locusts because of the relatively large size of the digestive tract and its powerful acid-base transporting capacities.

AB - This study examined the effect of temperature on hemolymph and intracellular acid-base status in the locust, Schistocerca nitens. Hemolymph pH decreased with temperature by 0.017 pH units·°C-1 above 25°C, but was stable at lower temperatures. Average intracellular pH (pHi, calculated from CO2 distribution) decreased by approximately 0.018 pH units·°C-1, in accordance with predictions for preservation of relative alkalinity and protein charge state. DMO was found to be unsuitable for use as an in vivo pHi marker in locusts due to rapid metabolism and excretion of this compound. Hemolymph pH regulation when temperature changed was accomplished by a combination of variation of PCO2 and [HCO3 -], with changes in [HCO3 -] predominating. Digestive tracts contained a large portion of total body water (over 30%), and total body CO2 (over 40%). Variation in [HCO3 -] may dominate pH regulation in locusts because of the relatively large size of the digestive tract and its powerful acid-base transporting capacities.

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