Temperature determines the diversity and structure of N2O-reducing microbial assemblages

Bo Wu, Feifei Liu, Michael D. Weiser, Daliang Ning, Jordan Okie, Lina Shen, Juan Li, Benli Chai, Ye Deng, Kai Feng, Liyou Wu, Shouwen Chen, Jizhong Zhou, Zhili He

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Micro-organisms harbouring the nosZ gene convert N2O to N2 and play a critical role in reducing global N2O emissions. As higher denitrifier diversity can result in higher denitrification rates, here we aimed to understand the diversity, composition and spatial structure of N2O-reducing microbial assemblages in forest soils across a large latitudinal and temperature gradient. We sequenced nosZ gene amplicons of 126 soil samples from six forests with mean annual soil temperatures (MAST) ranging from 3.7 to 25.3°C and tested predictions of the metabolic theory of ecology (MTE) and metabolic-niche theory (MNT). As predicted, α-diversity of nosZ communities increased with increasing MAST, within-site β-diversity decreased and two (pH and soil moisture) of the three niche widths examined were larger with increasing MAST. We calculated β-nearest taxon distance and Raup–Crick metric to quantify the relative influence of the assembly processes determining nosZ assemblage structure. Environmental selection was the primary process driving assemblage structure in all six forests. Homogenizing dispersal was also important at one site, which could be explained by the site's much lower variability in soil chemistry. We used canonical correspondence analysis and multiple regression on matrices to examine relationships between nosZ communities and environmental factors, and found that temperature and spatial distance were significant predictors of nosZ assemblage structure. Overall our results support both theories (MTE and MNT) tested, showing that higher temperatures are correlated with higher local diversity, wider niche breadths and lower within-site turnover rates. A plain language summary is available for this article.

Original languageEnglish (US)
Pages (from-to)1867-1878
Number of pages12
JournalFunctional Ecology
Volume32
Issue number7
DOIs
StatePublished - Jul 1 2018

Fingerprint

niches
soil temperature
niche
temperature
ecology
soil chemistry
niche breadth
denitrification
temperature profiles
forest soils
gene
latitudinal gradient
correspondence analysis
genes
soil sampling
temperature gradient
forest soil
soil water
multiple regression
microorganisms

Keywords

  • biogeography
  • diversity
  • latitudinal diversity gradient
  • NO-reducing community
  • nosZ
  • temperature gradient

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

Temperature determines the diversity and structure of N2O-reducing microbial assemblages. / Wu, Bo; Liu, Feifei; Weiser, Michael D.; Ning, Daliang; Okie, Jordan; Shen, Lina; Li, Juan; Chai, Benli; Deng, Ye; Feng, Kai; Wu, Liyou; Chen, Shouwen; Zhou, Jizhong; He, Zhili.

In: Functional Ecology, Vol. 32, No. 7, 01.07.2018, p. 1867-1878.

Research output: Contribution to journalArticle

Wu, B, Liu, F, Weiser, MD, Ning, D, Okie, J, Shen, L, Li, J, Chai, B, Deng, Y, Feng, K, Wu, L, Chen, S, Zhou, J & He, Z 2018, 'Temperature determines the diversity and structure of N2O-reducing microbial assemblages', Functional Ecology, vol. 32, no. 7, pp. 1867-1878. https://doi.org/10.1111/1365-2435.13091
Wu, Bo ; Liu, Feifei ; Weiser, Michael D. ; Ning, Daliang ; Okie, Jordan ; Shen, Lina ; Li, Juan ; Chai, Benli ; Deng, Ye ; Feng, Kai ; Wu, Liyou ; Chen, Shouwen ; Zhou, Jizhong ; He, Zhili. / Temperature determines the diversity and structure of N2O-reducing microbial assemblages. In: Functional Ecology. 2018 ; Vol. 32, No. 7. pp. 1867-1878.
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AU - Shen, Lina

AU - Li, Juan

AU - Chai, Benli

AU - Deng, Ye

AU - Feng, Kai

AU - Wu, Liyou

AU - Chen, Shouwen

AU - Zhou, Jizhong

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