TY - JOUR
T1 - Telomere shortening in cultured autografts of patients with burns
AU - Counter, Christopher M.
AU - Press, William
AU - Compton, Carolyn C.
N1 - Funding Information:
We thank all the patients and donors who provided us with samples, and R A Weinberg for his support. This work was supported by a grant from the NIH, administered though the NCI (CA82481 to C M Counter), by grant RO1-35242 from the NIH, and by grant 15891 from the Shriners' Hospital for Children (C C Compton). C M Counter is a Leukemia and Lymphoma Society Scholar. The funding source had no role in the study design, data collection, data analysis, data interpretation, or writing of the report.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2003/4/19
Y1 - 2003/4/19
N2 - In extensive third-degree burns, donor sites for conventional split thickness skin grafts are limited. In such cases, cultured epithelial (keratinocyte) grafts are prepared from small samples of the patient's own skin and expanded in tissue culture, a process that may incur very many cell divisions. Telomeres shorten with each cell division, and are markers of cellular proliferative history. We therefore measured telomere length in healed cultured epithelial autografts from four patients with burns, and noted that their telomeres were shorter than those in non-cultured skin from the same individuals, and than those in skin of healthy donors older than 80 years. Such great loss of telomeric DNA suggests that engrafted cells might have a shortened lifespan, which could have negative repercussions on the long-term viability of these grafts.
AB - In extensive third-degree burns, donor sites for conventional split thickness skin grafts are limited. In such cases, cultured epithelial (keratinocyte) grafts are prepared from small samples of the patient's own skin and expanded in tissue culture, a process that may incur very many cell divisions. Telomeres shorten with each cell division, and are markers of cellular proliferative history. We therefore measured telomere length in healed cultured epithelial autografts from four patients with burns, and noted that their telomeres were shorter than those in non-cultured skin from the same individuals, and than those in skin of healthy donors older than 80 years. Such great loss of telomeric DNA suggests that engrafted cells might have a shortened lifespan, which could have negative repercussions on the long-term viability of these grafts.
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U2 - 10.1016/S0140-6736(03)13042-5
DO - 10.1016/S0140-6736(03)13042-5
M3 - Article
C2 - 12711471
AN - SCOPUS:0037454399
SN - 0140-6736
VL - 361
SP - 1345
EP - 1346
JO - Lancet
JF - Lancet
IS - 9366
ER -