TbISWI regulates multiple polymerase I (Pol I)-transcribed loci and is present at Pol II transcription boundaries in Trypanosoma brucei

Tara M. Stanne, Manish Kushwaha, Matthew Wand, Jesse E. Taylor, Gloria Rudenko

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The unicellular eukaryote Trypanosoma brucei is unusual in having very little transcriptional control. The bulk of the T. brucei genome is constitutively transcribed by RNA polymerase II (Pol II) as extensive polycistronic transcription units. Exceptions to this rule include several RNA Pol I transcription units such as the VSG expression sites (ESs), which are mono-allelically expressed. TbISWI, a member of the SWI2/SNF2 related chromatin remodeling ATPases, plays a role in repression of Pol I-transcribed ESs in both bloodstream- and procyclic-form T. brucei. We show that TbISWI binds both active and silent ESs but is depleted from the ES promoters themselves. TbISWI knockdown results in an increase in VSG transcripts from the silent VSG ESs. In addition to its role in the repression of the silent ESs, TbISWI also contributes to the downregulation of the Pol I-transcribed procyclin loci, as well as nontranscribed VSG basic copy arrays and minichromosomes. We also show that TbISWI is enriched at a number of strand switch regions which form the boundaries between Pol II transcription units. These strand switch regions are the presumed sites of Pol II transcription initiation and termination and are enriched in modified histones and histone variants. Our results indicate that TbISWI is a versatile chromatin remodeler that regulates transcription at multiple Pol I loci and is particularly abundant at many Pol II transcription boundaries in T. brucei.

Original languageEnglish (US)
Pages (from-to)964-976
Number of pages13
JournalEukaryotic Cell
Volume10
Issue number7
DOIs
StatePublished - Jul 2011

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Trypanosoma brucei brucei
Histones
RNA Polymerase I
Chromatin Assembly and Disassembly
RNA Polymerase II
Eukaryota
Chromatin
Adenosine Triphosphatases
Down-Regulation
Genome

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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TbISWI regulates multiple polymerase I (Pol I)-transcribed loci and is present at Pol II transcription boundaries in Trypanosoma brucei. / Stanne, Tara M.; Kushwaha, Manish; Wand, Matthew; Taylor, Jesse E.; Rudenko, Gloria.

In: Eukaryotic Cell, Vol. 10, No. 7, 07.2011, p. 964-976.

Research output: Contribution to journalArticle

Stanne, Tara M. ; Kushwaha, Manish ; Wand, Matthew ; Taylor, Jesse E. ; Rudenko, Gloria. / TbISWI regulates multiple polymerase I (Pol I)-transcribed loci and is present at Pol II transcription boundaries in Trypanosoma brucei. In: Eukaryotic Cell. 2011 ; Vol. 10, No. 7. pp. 964-976.
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