Tailoring sub-micron PLGA particle release profiles via centrifugal fractioning

Dipankar Dutta, Mariama Salifu, Rachael W. Sirianni, Sarah Stabenfeldt

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Poly(D,L-lactic-co -glycolic) acid (PLGA)-based sub-micron particles are uniquely posed to overcome limitations of conventional drug delivery systems. However, tailoring cargo/payload release profiles from PLGA micro/nanoparticles typically requires optimization of the multi-parameter formulation, where small changes may cause drastic shifts in the resulting release profiles. In this study, we aimed to establish whether refining the average diameter of sub-micron particle populations after formulation alters protein release profiles. PLGA particles were first produced via double emulsion-solvent evaporation method to encapsulate bovine serum albumin. Particles were then subjected to centrifugal fractioning protocols varying in both spin time and force to determine encapsulation efficiency and release profile of differently sized populations that originated from a single batch. We found the average particle diameter was related to marked alterations in encapsulation efficiencies (range: 36.4-49.4%), burst release (range: 15.8-49.1%), and time for total cargo release (range: 38-78 days). Our data corroborate previous reports relating PLGA particle size with such release characteristics, however, this is the first study, to our knowledge, to directly compare particle population size while holding all formulation parameters constant. In summary, centrifugal fractioning to selectively control the population distribution of sub-micron PLGA particles represents a feasible tool to tailor release characteristics.

Original languageEnglish (US)
Pages (from-to)688-696
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume104
Issue number3
DOIs
StatePublished - Mar 1 2016

Fingerprint

Encapsulation
Population distribution
Refining
Emulsions
Evaporation
Particle size
Nanoparticles
Proteins
Acids
Bovine Serum Albumin
polylactic acid-polyglycolic acid copolymer
Milk
Drug Delivery Systems

Keywords

  • centrifugal fractioning
  • PLGA particles
  • protein encapsulation
  • release profile

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

Tailoring sub-micron PLGA particle release profiles via centrifugal fractioning. / Dutta, Dipankar; Salifu, Mariama; Sirianni, Rachael W.; Stabenfeldt, Sarah.

In: Journal of Biomedical Materials Research - Part A, Vol. 104, No. 3, 01.03.2016, p. 688-696.

Research output: Contribution to journalArticle

Dutta, Dipankar ; Salifu, Mariama ; Sirianni, Rachael W. ; Stabenfeldt, Sarah. / Tailoring sub-micron PLGA particle release profiles via centrifugal fractioning. In: Journal of Biomedical Materials Research - Part A. 2016 ; Vol. 104, No. 3. pp. 688-696.
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