Tailoring of the nanotexture of mesoporous silica films and their functionalized derivatives for selectively harvesting low molecular weight protein

Ye Hu, Ali Bouamrani, Ennio Tasciotti, Li Li, Xuewu Liu, Mauro Ferrari

Research output: Contribution to journalArticle

82 Scopus citations

Abstract

We present a fast, efficient, and reliable system based on mesoporous silica chips to specifically fractionate and enrich the low molecular weight proteome. Mesoporous silica thin films with tunable features at the nanoscale were fabricated using the triblock copolymer template pathway. Using different templates and concentrations in the precursor solution, various pore size distributions, pore structures, and connectivity were obtained and applied for selective recovery of low mass proteins. In combination with mass spectrometry and statistic analysis, we demonstrated the correlation between the nanophase characteristics of the mesoporous silica thin films and the specificity and efficacy of low mass proteome harvesting. In addition, to overcome the limitations of the prefunctionalization method in polymer selection, plasma ashing was used for the first time for the treatment of the mesoporous silica surface prior to chemical modification. Surface charge modifications by different functional groups resulted in a selective capture of the low molecular weight proteins from serum sample. In conclusion, our study demonstrates that the ability to tune the physicochemical properties of mesoporous silica surfaces, for a selective enrichment of the low molecular weight proteome from complex biological fluids, has the potential to promote proteomic biomarker discovery.

Original languageEnglish (US)
Pages (from-to)439-451
Number of pages13
JournalACS nano
Volume4
Issue number1
DOIs
StatePublished - Jan 26 2010

Keywords

  • Chemical modification
  • Early diagnostics
  • Low molecular weight proteome
  • Mass spectrometry
  • Mesoporous silica thin film
  • Nanotexture

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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