Systems-level understanding of ethanol-induced stresses and adaptation in E. coli

Huansheng Cao, Du Wei, Yuedong Yang, Yu Shang, Gaoyang Li, Yaoqi Zhou, Qin Ma, Ying Xu

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Understanding ethanol-induced stresses and responses in biofuel-producing bacteria at systems level has significant implications in engineering more efficient biofuel producers. We present a computational study of transcriptomic and genomic data of both ethanol-stressed and ethanol-adapted E. coli cells with computationally predicated ethanol-binding proteins and experimentally identified ethanol tolerance genes. Our analysis suggests: (1) ethanol damages cell wall and membrane integrity, causing increased stresses, particularly reactive oxygen species, which damages DNA and reduces the O 2 level; (2) decreased cross-membrane proton gradient from membrane damage, coupled with hypoxia, leads to reduced ATP production by aerobic respiration, driving cells to rely more on fatty acid oxidation, anaerobic respiration and fermentation for ATP production; (3) the reduced ATP generation results in substantially decreased synthesis of macromolecules; (4) ethanol can directly bind 213 proteins including transcription factors, altering their functions; (5) all these changes together induce multiple stress responses, reduced biosynthesis, cell viability and growth; and (6) ethanol-adapted E. coli cells restore the majority of these reduced activities through selection of specific genomic mutations and alteration of stress responses, ultimately restoring normal ATP production, macromolecule biosynthesis, and growth. These new insights into the energy and mass balance will inform design of more ethanol-tolerant strains.

Original languageEnglish (US)
Article number44150
JournalScientific reports
Volume7
DOIs
StatePublished - Mar 16 2017

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Systems-level understanding of ethanol-induced stresses and adaptation in E. coli'. Together they form a unique fingerprint.

  • Cite this