Systematic investigation of insulator-based protein dielectrophoresis under DC condition

A. Nakano, F. Camacho-Alanis, T. C. Chao, Alexandra Ros

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The ability to rapidly separate and analyze proteins in small volumes and concentrations is of great interest in situations where time is critical and sample availability is low. Here, we report on the dielectrophoretic manipulation of the proteins immunoglobulin G (IgG) and bovine serum albumin (BSA) under DC conditions in a tailored insulator-based microfluidic device. Our results demonstrate that dielectrophoresis can be used to concentrate protein monomers along a streamline profile. These findings are in excellent agreement with numerical simulations and indicate positive dielectrophoresis of proteins. Moreover, parameters such as conductivity and pH influencing protein dielectrophoresis were analyzed experimentally.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages644-646
Number of pages3
Volume1
StatePublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Fingerprint

Electrophoresis
Proteins
Microfluidics
Monomers
Availability
Computer simulation

Keywords

  • BSA
  • Dielectrophoresis
  • IgG
  • Insulator-based
  • Microfluidic

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Nakano, A., Camacho-Alanis, F., Chao, T. C., & Ros, A. (2011). Systematic investigation of insulator-based protein dielectrophoresis under DC condition. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 1, pp. 644-646)

Systematic investigation of insulator-based protein dielectrophoresis under DC condition. / Nakano, A.; Camacho-Alanis, F.; Chao, T. C.; Ros, Alexandra.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 1 2011. p. 644-646.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nakano, A, Camacho-Alanis, F, Chao, TC & Ros, A 2011, Systematic investigation of insulator-based protein dielectrophoresis under DC condition. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 1, pp. 644-646, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 10/2/11.
Nakano A, Camacho-Alanis F, Chao TC, Ros A. Systematic investigation of insulator-based protein dielectrophoresis under DC condition. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 1. 2011. p. 644-646
Nakano, A. ; Camacho-Alanis, F. ; Chao, T. C. ; Ros, Alexandra. / Systematic investigation of insulator-based protein dielectrophoresis under DC condition. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 1 2011. pp. 644-646
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