Synthetic Oligosaccharide Libraries and Microarray Technology: A Powerful Combination for the Success of Current Glycosaminoglycan Interactomics

Vitor H. Pomin, Xu Wang

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

Glycosaminoglycans (GAGs) are extracellular matrix and/or cell-surface sulfated glycans crucial to the regulation of various signaling proteins, the functions of which are essential in many pathophysiological systems. Because structural heterogeneity is high in GAG chains and purification is difficult, the use of structurally defined GAG oligosaccharides from natural sources as molecular models in both biophysical and pharmacological assays is limited. To overcome this obstacle, GAG-like oligosaccharides of well-defined structures are currently being synthesized by chemical and/or enzymatic means in many research groups around the world. These synthetic GAG oligosaccharides serve as useful molecular tools in studies of GAG–protein interactions. In this review, besides discussing the commonest routes used for the synthesis of GAG oligosaccharides, we also survey some libraries of these synthetic models currently available for research and discuss their activities in interaction studies with functional proteins, especially through the microarray approach.

Original languageEnglish (US)
Pages (from-to)648-661
Number of pages14
JournalChemMedChem
Volume13
Issue number7
DOIs
StatePublished - Apr 6 2018

Fingerprint

Microarrays
Glycosaminoglycans
Oligosaccharides
Technology
Library Surveys
Molecular Models
Research
Purification
Extracellular Matrix
Polysaccharides
Assays
Proteins
Pharmacology

Keywords

  • de novo synthesis
  • enzymatic synthesis
  • glycosaminoglycans
  • interactome
  • microarrays
  • oligosaccharides

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Drug Discovery
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Organic Chemistry

Cite this

Synthetic Oligosaccharide Libraries and Microarray Technology : A Powerful Combination for the Success of Current Glycosaminoglycan Interactomics. / Pomin, Vitor H.; Wang, Xu.

In: ChemMedChem, Vol. 13, No. 7, 06.04.2018, p. 648-661.

Research output: Contribution to journalReview article

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